Novus Biologicals, LLC
PO Box 802; Littleton, CO, 80160 USA
Phone: 303.730.1950 Fax: 303.730.1966
14-3-3 Antibody (NB100-2140)
14-3-3 Antibody
| Catalog#: | NB100-2140 |
This product is fully covered under guarantee+ |
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| Storage: | Store at 4C. Do not freeze. |
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| Buffer: | 0.2% Gelatin, PBS |
| Preservative: | 0.1% Sodium Azide |
| Purity: | Immunogen affinity purified |
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| Host: | Rb |
| Clonality: | Polyclonal |
| Specificity: | Broadly reactive among the 14-3-3 family. This recognizes alpha, beta, zeta (delta) and theta 14-3-3. |
| Modification: | No |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide mapping to the amino terminal domain of human 14-3-3. |
| Entrez: | 10971 (Human) |
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| Related Genes: | 10971 |
Background:
14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).
| Rt, Mu, Hu | |
| reactivity: | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.. |
| WB, IP, IHC | |||
| Uses: | This antibody is useful for Immunohistochemistry, Immunoprecipitation and Western Blot. | ||
| Dilutions: | immunohistochemistry , immunoprecipitation , Western Blot 1/400 | ||
| Positive Controls: | Hela Whole Cell Lysate (Hela Whole Cell Lysate (NB800-PC1) (1mg)) | Jurkat Whole Cell Lysate (Jurkat Whole Cell Lysate (NB800-PC2)(0.5mg)) | 14-3-3 Lysate (14-3-3 Lysate (NBL1-17951)(0.1mg)) |
| Publications: | Yaffe MB. 2002. How do 14-3-3 proteins work?- - Gatekeeper phosphorylation the molecular anvil hypothesis. FEBS Lett. 513(1):53-57. Liu MY, Cai S, Espejo A, Bedford MT, Walker CL. 2002. 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation sites. Cancer Res. 62(22): 6475-6480. Tzivion G, Shen YH, Zhu J. 2001. 14-3-3 proteins; bringing new definitions to scaffolding. Oncogene 20(44):6331-6338. |
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Reviews
No reviews have been submitted for this product.
Working with 14-3-3
Publications (4)
General:
Yaffe MB. 2002. How do 14-3-3 proteins work?- - Gatekeeper phosphorylation the molecular anvil hypothesis. FEBS Lett. 513(1):53-57.
Liu MY, Cai S, Espejo A, Bedford MT, Walker CL. 2002. 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation sites. Cancer Res. 62(22): 6475-6480.
Tzivion G, Shen YH, Zhu J. 2001. 14-3-3 proteins; bringing new definitions to scaffolding. Oncogene 20(44):6331-6338.
4 general publications available on website.
Images (1)
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