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Clonality:	Polyclonal
Specificity:	Broadly reactive among the 14-3-3 family. This recognizes alpha, beta, zeta (delta) and theta 14-3-3.
Purity:	Immunogen affinity purified
Host:	Rabbit

Immunogen:	Synthetic peptide mapping to the amino terminal domain of human 14-3-3.
Isotype:	IgG
Localization:	Cytoplasmic

Entrez:	10971 (Human)

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

	Rat, Mouse, Human
Reactivity:	Cross-reacts with Human, Mouse and Rat. Not yet tested in other species..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	WB, IP, IHC
Uses:	This antibody is useful for Immunohistochemistry, Immunoprecipitation and Western Blot.
Dilutions:	immunohistochemistry , immunoprecipitation , Western Blot 1/400
Positive Controls:	Hela Whole Cell Lysate (Hela Whole Cell Lysate (NB800-PC1)(1.0mg)) Jurkat Whole Cell Lysate (Jurkat Whole Cell Lysate (NB800-PC2)(0.5mg)) 14-3-3 Lysate (14-3-3 Lysate (NBL1-17951)(0.1mg))

Storage:	Store at 4C. Do not freeze.
Buffer:	0.2% Gelatin, PBS
Preservative:	0.1% Sodium Azide
Concentration:	0.2 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Yaffe MB. 2002. How do 14-3-3 proteins work?- - Gatekeeper phosphorylation the molecular anvil hypothesis. FEBS Lett. 513(1):53-57.
2. Liu MY, Cai S, Espejo A, Bedford MT, Walker CL. 2002. 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation sites. Cancer Res. 62(22): 6475-6480.
3. Tzivion G, Shen YH, Zhu J. 2001. 14-3-3 proteins; bringing new definitions to scaffolding. Oncogene 20(44):6331-6338.

4 publications available on website

 

2 Peptides and Proteins

6 Primary Antibodies

123 Secondary Antibodies

MAPT, TH, CSNK1E, AKT1, CSF2, LAMC2, RAF1, MAPK1, CDC25C, BCL2L1, BAD, HRAS1, MAPK3, KRAS, HCLS1, MAP3K5, PRNP, TNF, ZCCHC6, SPTB, GSTA4, ZHX2, CHEK1, APP, MAPK8, CASP3, PRKACA, CDK1, SNCA, ENO2, MAPK14, CDC2, SRC, BCL2, PRKCG, GRN, SGSM3, CDK5, INS, EGF