14-3-3 Antibody

Species: Rt, Mu, Hu Applications: WB, IP, IHC Clonality: Polyclonal	Gene: YWHAQ Purity: Immunogen affinity purified Host: Rabbit
Specificity: Broadly reactive among the 14-3-3 family. This recognizes alpha, beta, zeta (delta) and theta 14-3-3. Publications: Antibody has not yet been mentioned in a publication.
See All 14-3-3 Products See All 14-3-3 Related Products: Peptides and Proteins Primary Antibodies
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Isotype:	IgG
Immunogen:	Synthetic peptide mapping to the amino terminal domain of human 14-3-3.
Localization:	Cytoplasmic

Entrez:	10971 (Human)
Related Genes:	MAPT, TH, CSNK1E, AKT1, CSF2, LAMC2, RAF1, MAPK1, CDC25C, BCL2L1, BAD, HRAS1, MAPK3, KRAS, HCLS1, MAP3K5, PRNP, TNF, ZCCHC6, SPTB, GSTA4, ZHX2, CHEK1, APP, MAPK8, CASP3, PRKACA, CDK1, SNCA, ENO2, MAPK14, CDC2, SRC, BCL2, PRKCG, GRN, SGSM3, CDK5, INS, EGF

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Reactivity:	Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Uses:	This antibody is useful for Immunohistochemistry, Immunoprecipitation and Western Blot.
Dilutions:	immunohistochemistry , immunoprecipitation , Western Blot 1/400
Concentrations:	0.2 mg/ml
Positive Controls:	3 positive controls

Buffer:	0.2% Gelatin, PBS
Storage:	Store at 4C. Do not freeze.
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.