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Clonality:	Polyclonal
Specificity:	This may crossreact with ACTR1B
Purity:	Immunogen affinity purified
Host:	Goat

Immunogen:	Synthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727.
Isotype:	IgG
Localization:	Cytoplasmic

Entrez:	10121 (Human)
	54130 (Mouse)
Swiss Prot:	NP_005727. (Human)

The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.

	Human
Reactivity:	Human, mouse and rat. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	WB, PEP-ELISA
Uses:	This antibody is useful for Peptide ELISA and Western Blot. Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 42.6kDa according to NP_005727.1).
Dilutions:	Peptide ELISA 1:1000, Western Blot 1-3 ug/ml
Positive Controls:	Whole Normal Brain Lysate (Whole Normal Brain Lysate (NB820-59177)(1.0mg)) ACTR1A Lysate (ACTR1A Lysate (NBL1-07285)(0.1mg))

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-saline (pH7.3) with 0.5% BSA
Preservative:	0.02% Sodium Azide
Concentration:	0.5 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992).

 

Western Blot: ARP1 homolog A Antibody [NB100-1423]- staining (2ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour.

1 Lysates

4 Peptides and Proteins

3 Primary Antibodies

1 RNAi

95 Secondary Antibodies

NR2F2, DCTN1, ARFRP1, IKBKAP, ABL1, CHAF1A, PIK3R4, ANPEP, CTR9, RAB3GAP2, AKAP5, NR2F1, ANGPTL1, APOBEC2, BACE2, ANGPTL2, ACTR2, AICDA, KAP, SQSTM1, NUP62, CRISP1, AKR1B10, KHDRBS1, VCSA1, IGF2BP2, GTF2H1, MANF, RERE, DCTN4, ANGPTL4, APOBEC3B, ACTL6A, ACTR3, ACTR1B, TPPP, ACTB, CD40, LSP1, ARHGEF7