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ACTR1A Antibody (NB100-1423)

ACTR1A Antibody

Catalog#: NB100-1423 100% Guarantee
This product is fully covered under guarantee+
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer: Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA
Preservative: 0.02% Sodium Azide
Purity: Immunogen affinity purified
Host: Gt
Clonality: Polyclonal
Specificity: This may crossreact with ACTR1B
Modification: No
Isotype: IgG
Immunogen: Synthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727.
Gene Symbol: ACTR1A 
Entrez: 10121 (Human)
54130 (Mouse)
Related Genes: 10121
Swiss Prot: NP_005727. (Human)
Background:
The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.
Species:
Hu
reactivity: Human, mouse and rat. .
Applications:
WB, PEP-ELISA
Uses: This antibody is useful for Peptide ELISA and Western Blot. Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 42.6kDa according to NP_005727.1).
Dilutions: Peptide ELISA 1:1000, Western Blot 1-3 ug/ml
Positive Controls: Whole Normal Brain Lysate (Whole Normal Brain Lysate (NB820-59177)(1.0mg)) ACTR1A Lysate (ACTR1A Lysate (NBL1-07285)(0.1mg))
Publications: Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992).
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Working with ACTR1A

Publications (1)
General:

Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992).


 
Images (1)
Western Blot: ARP1 homolog A Antibody [NB100-1423]- staining (2ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Western Blot: ARP1 homolog A Antibody [NB100-1423]- staining (2ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour.