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Clonality:	Monoclonal
Specificity:	This is specific for HIF-1 alpha.
Purity:	protein G purified
Host:	Mouse

Immunogen:	Human HIF-1 alpha amino acids 329-530.
Clone:	ESEE122
Isotype:	IgG1
Localization:	Nuclear

Entrez:	3091 (Human)
	15251 (Mouse)
	29560 (Rat)
Swiss Prot:	Q16665 (Human)

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.

	Rat, Mouse, Human, Bovine
Reactivity:	NB 100-131 recognizes human, bovine, mouse and rat HIF-1 alpha. .
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	IHC-Fr, IHC-P, ICC, IP, IF, IHC
Uses:	Works well on most histochemical applications. NOT recommended specifically for Western analysis, although some people have used it and prefer it for Western Blot. Procedure: http://ihcworld.com/_protocols/antibody_protocols/hif_1alpha_novus.htm
Dilutions:	immunohistochemistry 1:100-1:5000, immunofluorescence , immunoprecipitation , Immunocytochemistry , Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen ,
Conjugated Ab's:	6 conjugated antibodies
Positive Controls:	RCC4 Lysate (RCC4 Lysate (NBP1-30412)(1.0mg)) COS-7 [Hypoxia induced and uninduced Nuclear Extract Kit] Lysate (COS-7 Lysate (NB800-PC26)(4 vials))

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Protein G purified mouse ascites
Preservative:	0.01% Sodium Azide
Unit Size:	0.1ml (also available: 0.025ml (sample size))
Concentration:	1.0 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. Ðordevic G., Matušan-Ilijaš K., Babarovic E., et al. Hypoxia inducible factor-1a correlates with vascular endothelial growth factor A and C indicating worse prognosis in clear cell renal cell carcinoma. Journal of Experimental & Clinical Cancer Research 2009, 28:40 [PMID: 19302703] (IHC-P, Human)
2. Abbate A, Santini D, Biondi-Zoccai GG, et al. Cyclo-oxygenase-2 (COX-2) expression at the site of recent myocardial infarction: friend or foe? Heart. 2004 Apr;90(4):440-3. [PMID: 15020525] (IHC-P, human)
3. Khan Z, Michalopoulos GK, Stolz DB. Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation. Am J Pathol. 2006 Oct;169(4):1251-69. [PMID: 17003483] (ICC/IF, Rat)

16 publications available on website

Application: IHC-P on 02/02/2009

Images (6)

	Iummunohistochemistry: HIF-1 alpha (ESEE122) [NB100-131] - HIF-1 Alpha staining in hypoxia-induced human placenta using NB 100-131.		Immunohistochemistry-paraffin: HIF-1 alpha (ESEE1220 [NB100-131] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
	Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. Blue dye is DAPI nuclear staining. Photos courtesy of		Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclear staining.
	Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.		Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

3 Antibody Packs

1 Antibody Pairs

1 Lysates

4 Peptides and Proteins

62 Primary Antibodies

2 RNAi

216 Secondary Antibodies

VEGFA, SETD2, ARNT, EPO, VHL, EPAS1, AKT1, MAPK3, SLC2A1, MIA3, TRP53, NOS2, CA9, TP53, PTGS2, MAPK1, KDR, TNF, EPX, EGLN1, JUN, MTOR, PIK3R1, EP300, PIK3CG, PIK3CB, NFKB1, NOTCH1, BCL2, SLC1A3, HMOX1, IL6, CDKN1A, CASP3, MMP2, HIF1AN, CAR9, EGFR, PSMC6, MMP9

General Protocols

• Protocol specific for HIF-1 alpha Antibody (NB100-131)

FAQs (3) (Available Online)

• Can NB100-131 be used to perform immuno-fluorescent staining at specific tissues?
• What is a good positive control for detecting HIF-1 alpha in immunohistochemistry?
• How do you induce HIF-1 alpha in samples?