Mre11 Antibody (NB100-142)
Mre11 Antibody
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anti-Ataxia-telangiectasia disorder-like antibody, anti-MRE11b antibody, Anti-MRE11a antibody, anti-MRE 11b antibody, Anti-MRE 11a antibody, anti-Mre 11 antibody, anti-Meiotic Recombination 11 Homolog A antibody, anti-Double-strand break repair protein MRE11A antibody, anti-ATLD antibody, |
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Mre11 Antibody Summary: | | Gene: | MRE11A |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | This is specific for human Mre11. |
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| Publications: | Antibody has been mentioned in at least 68 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Mre11 Products Mre11 Antibody Details: | Immunogen: | Full length human Mre11 protein. |
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100-142 recognizes hamster, human and mouse. Other species have not been tested. |
| Uses: | Western Blot, histochemical applications, immunoprecipitation. By Western blot, this Mre11 antibody recognizes a band at 81 kDa, representing hMre11 in TK6 or other human cells. By immunofluorescence, it has been used with methanol-fixed IMR90 primary human fibroblasts and has been used in the literature for IP as well. The suggested working diultion for IP is 3 ul for immunoprecipitation of 3X10^6 cells. Has also been used for immunohistochemistry on paraffin sections. |
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| Dilutions: | Immunocytochemistry, Immunofluorescence 1:200, Immunohistochemistry-Paraffin, Immunoprecipitation 3 ul, Western Blot 1:5000 |
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| Unit Size: | 0.05 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 3 Positive Controls |
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Packaging: | Storage: | Store short term at 4 °C. Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 0.02 % Sodium Azide |
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| Preservative: | Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA-Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions. This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity.
HMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway. However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites. These findings are consistent with the distinct role of these proteins in DNA repair.
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Images (3) Reviews (0) Related Products (19) Exploring Mre11 Working with Mre11
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with Mre11
Mre11 Antibody Images (3) Immunocytochemistry/Immunofluorescence: Mre11 Antibody [NB100-142] - Detection of MRE11 (Green) in Hela cells using NB100-142 at a 1:200 dilution. Nuclei (Blue) are counterstained using Hoechst 33258. |  Western Blot: Mre11 Antibody [NB100-142] - Western Blot analysis of Mre11 on 50 ug of HeLa and MEF lysates, using NB100-142 |  Immunohistochemistry: Mre11 antibody [NB100-142] - MRE11 staining in the human epidermis detected with NB100-142. |
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Publications (68)Mre11 Antibody Product Specific:- Cheng CY, Gilson T, Dallaire F, et al. The E4orf6/E1B55K E3 ubiquitin ligase complexes of human adenoviruses exhibit heterogeneity in composition and substrate specificity. J. Virol. 2011;85(2):765-775.[PMID : 21068234]
- Kaul Z, Cesare AJ, Huschtscha LI, et al. Five dysfunctional telomeres predict onset of senescence in human cells. EMBO Rep. 2011 Dec 23;13(1):52-9. [PMID 22157895]
more... - Mohapatra S, Kawahara M, Khan IS, et al. Restoration of G1 chemo/radioresistance and double-strand-break repair proficiency by wild-type but not endonuclease-deficient Artemis. Nucleic Acids Res. 2011 Aug;39(15):6500-10. [PMID 21531702]
- Coleman KA, Greenberg RA. The BRCA1-RAP80 complex regulates DNA repair mechanism utilization by restricting end resection. J Biol Chem. 2011 Feb 18. [PMID: 21335604]
- Schmid M, Kindsmüller K, Wimmer P, et al. The E3 Ubiquitin Ligase Activity Associated with the Adenoviral E1B-55K-E4orf6 Complex Does Not Require CRM1-Dependent Export. J. Virol. 2011 Jul.[PMID 21561915]
- Schreiner S, Wimmer P, Groitl P, et al. Adenovirus type 5 early region 1B 55K oncoprotein dependent degradation of cellular factor Daxx is required for efficient transformation of primary rodent cells. J Virol. 2011 Jun 22.[PMID: 21697482]
- Matsumoto Y, Miyamoto T, Sakamoto H, et al. Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly.DNA Repair (Amst). 2011 Jan 10. [PMID: 21227757]
- Splinter J, Jakob B, Lang M, et al. Biological dose estimation of UVA laser microirradiation utilizing charged particle-induced protein foci. Mutagenesis. 2010 May;25(3):289-97. [PMID: 20167590] (Human, IF/ICC)
- S. Schreiner, P. Wimmer, H. Sirma, et al. Proteasome-dependent degradation of Daxx by the viral E1B-55K protein in human adenovirus-infected cells. J. Virol. 2010; JVI.00074-10. [PMID: 20484509]
- Dallaire F, Blanchette P, Branton PE. A proteomic approach to identify candidate substrates of human adenovirus E4orf6-E1B55K and other viral cullin-based E3 ubiquitin ligases. J Virol. 2009 Dec;83(23):12172-84. [PMID: 19759146]
- Yan T, Seo Y, Kinsella TJ. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res. 2009 Nov 15;15(22):6912-20. [PMID: 19861440]
- Kindsmuller K, Schreiner S, Leinenkugel F, et al. A 49-Kilodalton Isoform of the Adenovirus Type 5 Early Region 1B 55-Kilodalton Protein Is Sufficient To Support Virus Replication. J Virol 2009;83(18):9045-9056. [PMID: 19587039]
- Yu Z, Chen T, Hebert J, et al. A Mouse PRMT1 Null Allele Defines an Essential Role for Arginine Methylation in Genome Maintenance Cell Proliferation. Mol Cell Biol 2009;29(11):2982-2996. [PMID: 19289494]
- Dallaire F, Blanchette P, Groitl P, et al. Identification of Integrin {alpha}3 as a New Substrate of the Adenovirus E4orf6/E1B 55-Kilodalton E3 Ubiquitin Ligase Complex. J Virol 2009;83(11):5329-5338. [PMID: 19297475]
- Shao L, Fujii H, Colmegna I, et al. Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis. J Exp Med 2009:jem.20082251. [PMID: 19451263]
- M Shimada, R Sagae, J Kobayashi, et al. Inactivation of the Nijmegen Breakage Syndrome Gene Leads to Excess Centrosome Duplication via the ATR/BRCA1 Pathway. Cancer Res. 2009 Mar 1;69(5):1768-75. [PMID: 19244116]
- Nahas SA, Butch AW, Du L, et al. Rapid Flow Cytometry-Based Structural Maintenance of Chromosomes 1 (SMC1) Phosphorylation Assay for Identification of Ataxia-Telangiectasia Homozygotes Heterozygotes. Clin Chem 2009:clinchem.2008.107128 [PMID: 19147735].
- Karen KA, Hoey PJ, Young CSH, et al. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol. 2009 May;83(9):4565-73. [PMID: 19244322]
- Bencokova Z, Kaufmann MR, Pires IM, et al. ATM activation and signaling under hypoxic conditions. Mol Cell Biol. 2009 Jan;29(2):526-37. Epub 2008 Nov 3.
- Ewald B, Sampath D, Plunkett W. ATM and the Mre11-Rad50-Nbs1 complex respond to nucleoside analogue-induced stalled replication forks and contribute to drug resistance. Cancer Res. 2008 Oct 1;68(19):7947-55.
- Guirouilh-Barbat J, Redon C, Pommier Y. Transcription-coupled DNA double-strand breaks are mediated via the nucleotide excision repair and the Mre11-Rad50-Nbs1 complex. Mol Biol Cell. 2008 Sep;19(9):3969-81. Epub 2008 Jul 16.
- Bouska A, Lushnikova T, Plaza S, et al. Mdm2 promotes genetic instability and transformation independent of p53. Mol Cell Biol. 2008 Aug;28(15):4862-74. Epub 2008 Jun 9.
- Ullman AJ, Hearing P. Cellular proteins PML and Daxx mediate an innate antiviral defense antagonized by the adenovirus E4 ORF3 protein. J Virol. 2008 Aug;82(15):7325-35. Epub 2008 May 14.
- Blackford AN, Bruton RK, Dirlik O, et al. A role for E1B-AP5 in ATR signaling pathways during adenovirus infection. J Virol. 2008 Aug;82(15):7640-52. Epub 2008 May 14.
- Zhao X, Madden-Fuentes RJ, Lou BX, et al. Ataxia telangiectasia-mutated damage-signaling kinase- and proteasome-dependent destruction of Mre11-Rad50-Nbs1 subunits in Simian virus 40-infected primate cells. J Virol. 2008 Jun;82(11):5316-28. Epub 2008 Mar 19.
- Zhong Z-H, et al. Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem. 2007 Oct 5;282(40):29314-22. Epub 2007 Aug 9. (Western Blot)
- Lin JJ, Dutta A. ATR pathway is the primary pathway for activating G2/M checkpoint induction after re-replication. J Biol Chem. 2007 Oct 19;282(42):30357-62. Epub 2007 Aug 22.
- Blanchette P, Kindsmuller K, Groitl P, et al. Control of mRNA export by adenovirus E4orf6 and E1B55K proteins during productive infection requires E4orf6 ubiquitin ligase activity. J Virol. 2008 Mar;82(6):2642-51. Epub 2008 Jan 9.
- Kruger, L., et al. Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein. Carcinogenesis. 2007 Jan;28(1):107-11. Epub 2006 Jul 13.
- Stewart, G.S. et al. RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16910-5. Epub 2007 Oct 16.
- Takemura, Haruyuki., et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006 Oct 13;281(41):30814-23. Epub 2006 Aug 10.
- Cahill, Dana, et al. Dimerization of the Rad50 protein is independent of the conserved hook domain. Mutagenesis. 2007 Jul;22(4):269-74. Epub 2007 Apr 9.
- Varon, R., et al. Mild Nijmegen breakage syndrome phenotype due to alternative splicing. Hum Mol Genet. 2006 Mar 1;15(5):679-89. Epub 2006 Jan 13.
- Seemanova, E., et al. Nijmegen breakage syndrome (NBS) with neurological abnormalities and without chromosomal instability. J Med Genet. 2006 Mar;43(3):218-24. Epub 2005 Jul 20.
- Cerosaletti, K., et al. Active role for nibrin in the kinetics of atm activation. Mol Cell Biol. 2006 Mar;26(5):1691-9.
- Myers, J.S., ; Cortez, D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem. 2006 Apr 7;281(14):9346-50. Epub 2006 Jan 23.
- Bekker-Jensen, S., et al. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks. J Cell Biol. 2006 Apr 24;173(2):195-206. Epub 2006 Apr 17.
- Sheen, M., et al. Mre11-Rad50-Nbs1 complex is activated by hypertonicity. Am J Physiol Renal Physiol. 2006 Nov;291(5):F1014-20. Epub 2006 Jun 20.
- Yi, W., et al. Physical and functional interaction between hMSH5 and c-Abl. Cancer Res. 2006 Jan 1;66(1):151-8.
- Alt, J.R., et al. Mdm2 binds to Nbs1 at sites of DNA damage and regulates double strand break repair. J Biol Chem. 2005 May 13;280(19):18771-81. Epub 2005 Feb 25. [Western Blot, Human]
- Brown, M.L., et al. Metabolism of recombination coding ends in scid cells. J Immunol. 2000 Apr 15;164(8):4135-42. [Western Blot, Mouse]
- Kracker, S. et al. Nibrin functions in Ig class-switch recombination. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1584-9. Epub 2005 Jan 24. [Mouse]
- Mohammadi, E., et al. Expression of the adenovirus E4 34k oncoprotein inhibits repair of double strand breaks in the cellular genome of a 293-based inducible cell line. Nucleic Acids Res. 2004 May 11;32(8):2652-9. Print 2004. [Flow Cytometry, Western Blot, Immunofluorescence, Human]
- Cerosaletti, K., et al. Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm. J Biol Chem. 2004 Sep 10;279(37):38813-9. Epub 2004 Jul 1. [Western Blot, Immunofluorescence, Human]
- Robison, J., et al. Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks. J Biol Chem. 2004 Aug 13;279(33):34802-10. Epub 2004 Jun 4. [Western Blot, Immunofluorescence, Human]
- Sedelnikova, O., et al. Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks. Nat Cell Biol. 2004 Feb;6(2):168-70. [Mouse]
- Li, Y., et al. Notch and Schwann cell transformation. Oncogene. 2004 Feb 5;23(5):1146-52. [Human]
- Cheng, W., et al. Linkage between Werner syndrome protein and the Mre11 complex via Nbs1. J Biol Chem. 2004 May 14;279(20):21169-76. Epub 2004 Mar 16. [Immunoprecipitation, Immunofluorescence, Western Blot, Human]
- Udayakumar, D., et al. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003 Oct 24;278(43):41631-5. Epub 2003 Aug 12.
- Blanchette, P., et al. Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53. Mol Cell Biol. 2004 Nov;24(21):9619-29.
- Furuta, T., et al. Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. J Biol Chem. 2003 May 30;278(22):20303-12. Epub 2003 Mar 25. [Western Blot, Human]
- Wu, G., et al. NBS1 and TRF1 colocalize at promyelocytic leukemia bodies during late S/G2 phases in immortalized telomerase-negative cells. Implication of NBS1 in alternative lengthening of telomeres. J Biol Chem. 2000 Sep 29;275(39):30618-22.
- Donahue, S., et al. A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts. Nucleic Acids Res. 2004 Jun 15;32(10):3248-57. Print 2004.
- Donahue, S., et al. Deficient regulation of DNA double-strand break repair in Fanconi anemia fibroblasts. J Biol Chem. 2003 Aug 8;278(32):29487-95. Epub 2003 May 14.
- Siwicki, J., et al. Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients. Exp Cell Res. 2003 Jul 1;287(1):178-89.
- Cerosaletti, K., et al. Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation. J Biol Chem. 2003 Jun 13;278(24):21944-51. Epub 2003 Apr 4.
- Limoli, C.L., et al. DNA replication arrest in XP variant cells after UV exposure is diverted into an Mre11-dependent recombination pathway by the kinase inhibitor wortmannin. Mutat Res. 2002 Dec 29;510(1-2):121-9.
- Kobayashi, J., et al. NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain. Curr Biol. 2002 Oct 29;12(21):1846-51.
- Her, C., et al. Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1. DNA Repair (Amst). 2002 Sep 4;1(9):719-29.
- Cleaver, J.E., et al. Polymerase eta and p53 jointly regulate cell survival, apoptosis and Mre11 recombination during S phase checkpoint arrest after UV irradiation. DNA Repair (Amst). 2002 Jan 22;1(1):41-57.
- Digweed M, Demuth I, Rothe S, et al. SV40 large T-antigen disturbs the formation of nuclear DNA-repair foci containing MRE11. Oncogene. 2002 Jul 25;21(32):4873-8. [PMID: 12118365] (Human, ICC)
- Carbone R, Pearson M, Minucci S, et al. PML NBs associate with the hMre11 complex and p53 at sites of irradiation induced DNA damage. Oncogene. 2002 Mar 7;21(11):1633-40. [PMID: 11896594]
- Brown, M.L., et al. Role of poly(ADP-ribosyl)ation in DNA-PKcs- independent V(D)J recombination. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4532-7.
- Limoli CL, Giedzinski E, Bonner WM, et al. UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-strand breaks, gamma -H2AX formation, and Mre11 relocalization. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):233-8. [PMID: 11756691] (Human, IF)
- Limoli CL, Giedzinski E, Morgan WF, et al. Polymerase eta deficiency in the xeroderma pigmentosum variant uncovers an overlap between the S phase checkpoint and double-strand break repair. Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7939-46. [PMID: 10859352] (Human, IF)
- Franchitto A, Pichierri P. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol. 2002 Apr 1;157(1):19-30. [PMID: 11916980] (Human, IF, IP, WB)
- Tauchi H, Kobayashi J, Morishima K, et al. The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50/hMRE11/NBS1 complex DNA repair activity. J Biol Chem. 2001 Jan 5;276(1):12-5. [PMID: 11062235] (Chicken, WB, IF)
- Wang Y, Cortez D, Yazdi P, et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000 Apr 15;14(8):927-39. [PMID: 10783165] (Human, WB, IP, IF)
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