Mre11 Antibody (12D7) (NB100-473)

Mre11 Antibody (12D7) (NB100-473) (0.1 mg)

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	Catalog Number	NB100-473
	Size(s)	0.1 mg	Price:
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anti-endo/exonuclease Mre11 antibody, anti-MRE11 homolog 1 antibody, anti-double-strand break repair protein MRE11A antibody, anti-HNGS1 antibody, anti-DNA recombination and repair protein antibody, anti-Meiotic recombination 11 homolog A antibody, anti-AT-like disease antibody, anti-Meiotic recombination 11 homolog 1 antibody, anti-MRE11MRE11B antibody, anti-ATLDmeiotic recombination (S. cerevisiae) 11 homolog A antibody, anti-MRE11 homolog A antibody, anti-MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody

Mre11 Antibody (12D7) Summary:

Species:	Hu	Gene:	MRE11A
Applications:	WB, ELISA, IF, IHC-P, IP	Purity:	Protein G purified
Clonality:	Monoclonal	Host:	Mouse
Publications:	NB100-473 has been mentioned in at least 19 Publications

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Lysates	Peptides and Proteins
Primary Antibodies	RNAi
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Mre11 Antibody (12D7) Details:

Clone:	12D7
Isotype:	IgG1
Immunogen:	Amino acids 182-582 of Mre11 expressed in e. coli.
Localization:	Nuclear

Species Reactivity:

Cross-reacts with Human. Not yet tested in other species.

Applications:

Uses:	For IHC, incubation with the primary antibody was preceded by microwave treatment of the tissue section for antigen retrieval.
Dilutions:	ELISA, Immunofluorescence 1:1001:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation 1:100, Western Blot 1:1000-1:2000
MW:	81 kDa
Unit Size:	0.1 mg
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer:	PBS, pH 7.4
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MRE11A

Entrez:	4361 (Human)
Uniprot:	P49959 (Human)

Related Genes:

Background:

Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3'-5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex with Rad50, p95 (nibrin) and possibly other associated proteins involved in the nuclelytic end-processing of double strand breaks and telomere maintenance.

Images (2)


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Mre11 Antibody (12D7) Images (2)

	Western Blot: Mre11 Antibody (12D7) [NB100-473] - Analysis of human MRE11 expression by western blot in HeLa (1) and Raji (2) whole cell extracts using anti-MRE11 [12D7] monoclonal antibody (NB100-473).

	Immunofluorescent: Mre11 Antibody (12D7) [NB100-473] - Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells. (Panel D - negative control). Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

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Publications (19)

Mre11 Antibody (12D7) Product Specific:

Giannini, G., et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene. 23:2640-2647, 2004.
Stracker, T.H. et al. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature. 418:348-352, 2002.

more...

Uziel, T. et al. Requirement of the MRN complex for ATM activation by DNA damage. EMBO J. 22:5612-5621, 2003.
Xu, X., Stern. D.F. NFBD1/MDC1 refulates ionizing rediation-induced focus formation by DNA checkpoint signaling repair factors. FASEB. 17:1842-1848, 2003.
Celeste, A. et al. Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Nature Cell Biol. 5:675-679,
Gatei, M. et al. ATM-dependent phosphorylation of nibrin in response to radiation exposure. Nature Genetics. 25:115-119, 2000.
Lukas, C. et al. Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nature Cell Biol. 5:255-260, 2003.
Nakanishi, K. et al. Interaction of FANCD2 NBS1 i nthe DNA damage response. Nature Cell Biol. 4:913-920, 2002.
Stewart, G.S. et al. MDC! is a mediator of the mammalian DNA damage checkpoint. Nature. 421:961-966, 2003.
Wang, Y. et al. BASC, a super complex of BRCA1-associated proteins is involved in the recognition repair of aberrant DNA structures. Genes Dev. 14:927-939, 2000.
Lombard, D.B. et al. Nijmegen breakage syndrome disease protein Mre11 at PML nuclear bodies meiotic telomeres. Cancer Res. 60:2331-2334, 2000.
Limoli, C.L. et al. UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-str breaks, gamma-H2AX formation, Mre11 relocalization. PNAS. 99:233-238, 2002.
Lee, H. et al. Distinct functions of nijmegen breakage syndromw in ataxia telangiectasia mutated-dependent responses to DNA damage. Mol. Cancer Res.
Xu, X. et al. Is a chromatin-associeated protein involved in DNA damage signaling pathways. J. Biol. Chem. 278:8795-8803, 2003.
Giannini G et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene 23:2640-7 (2004).
Zheng L, Kanagaraj R, Mihaljevic B, et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 2009;37(8):2645-2657. [pubmed: 19270065]
Helmink BA, Bredemeyer AL, Lee B-S, et al. MRN complex function in the repair of chromosomal Rag-mediated DNA double-str breaks. J Exp Med 2009;206(3):669-679 [pubmed: 19221393]
Helmink BA, Bredemeyer AL, Lee B-S, et al. MRN complex function in the repair of chromosomal Rag-mediated DNA double-str breaks. J Exp Med. 2009 Mar 16;206(3):669-79. [pubmed: 19221393]
Ottini, L., et al. MRE11 expression is impaired in gastric cancer with microsatellite instability. Carinogenesis. 25: 2337-2343, 2004.

Protocols

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Other Positive Controls T24 cells

Lysates (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Mre11 Lysate	NBL1-13219		WB	(1)

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Mre11 Recombinant Protein	H00004361-P01	Hu	ELISA, WB	(1)
Mre11 Peptide	NBP1-77094PEP	Hu	B/N

Primary Antibodies (13)

Name	Catalog#	Reactivity	Applications	Images	Publications
Mre11 Antibody	NB100-142	Ha, Hu, Mu	ICC, IF, IHC-P, IP, WB	(3)	(76)
Mre11 Antibody	NBP1-90074	Hu	IF, IHC-P, WB	(3)
Mre11 Antibody	NB100-276	Hu	IHC-P, WB	(2)
Mre11 Antibody	NBP1-77094	Hu, Mu, Rt	ELISA, IHC-P, WB	(2)
Mre11 Antibody	NBP1-19528	Hu, Mu, Rt	IHC-P, WB	(2)
... and 8 more. See All »

RNAi (2)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Mre11 RNAi	H00004361-R01	Hu		(1)	(4)
Mre11 RNAi	H00004361-R02	Hu		(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Publications
Mouse IgG Antibody [Biotin]	NB720-B	Mu	ELISA, IHC-P, WB
Mouse IgG Antibody [HRP]	NB720-H	Mu	ELISA, ICC, IHC-P, WB
Mouse IgG Antibody [Alkaline Phosphatase]	NB720-AP	Mu	ELISA, ICC, IHC-P, WB	(1)
Mouse IgG Antibody [FITC]	NB720-F	Mu	ICC, IHC-P	(2)
Mouse IgG Antibody	NB120-7056	Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)	ELISA, IHC-P, IP, WB
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Mre11 in Context

Research Areas related to Mre11 (2):