Mouse IgG1, kappa L Chain Isotype Control (NBA1-21302)

Mouse IgG1, kappa L Chain Isotype Control

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Mouse IgG1, kappa L Chain Isotype Control Summary:
Species:Mu
Applications:IA, WB
Purity:IgG purified
Host:Mouse
Specificity:This mouse IgG1 immunoglobulin is useful as an isotype control for mouse IgG1 antibodies.
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Mouse IgG1, kappa L Chain Isotype Control Details:
Isotype:IgG1 Kappa
Immunogen:N/A
Species Reactivity:

This is an isotype control antibody.

Applications:
Uses:Used as a positive control in immunoassays.
Dilutions:Western Blot , Immunoassay
Unit Size:0.2 ml
Concentration:Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:0.0 M Tris, 0.5 M NaCl, pH 7 7.
Preservative:0.1% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Background:

Mouse IgG1 myeloma cells were grown in mice and ascitic fluids collected. The IgG1 was purified by a combination of salt fractionation, ion exchange and protein A chromatography. Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis the IgG1 was shown to 1) react with antiserum specific for mouse IgG1, 2) not react with antiserum specific for other immunoglobulins, and 3) produce a single precipitation arc with antiserum against mouse serum identical to that produced with anti-IgG1.

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Publications (1)

Mouse IgG1, kappa L Chain Isotype Control Product Specific:

  1. Ferno et al., Vaccine 13 1460-1467, 1995.

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