By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human IgG was detected.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
ELISA, Immunohistochemistry-Paraffin, Western Blot and Immunocytochemistry
Dilutions:
ELISA coating-1:50-1:250 Primary-1:1000-1:10000, Immunocytochemistry 1:100 - 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:1000, Western Blot colorimetric-1:500-1:5000 Chemiluminescent 1:500-1:15000
Unit Size:
0.5 mg
Concentration:
0.5 mg/ml
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
phosphate buffered saline, pH 7.2
Preservative:
0.1% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.