Goat anti-Mouse IgG (H+L) Secondary Antibody [HiLyte Fluor 680] Summary
| Immunogen |
Mouse IgG |
| Specificity |
Mouse IgG (H+L) |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Goat |
| Purity |
Immunogen affinity purified |
Applications/Dilutions
| Dilutions |
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence
|
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
0.01 mM phosphate, 150 mM NaCl, pH 7.2, with bovine serum albumin. |
| Preservative |
2mM Sodium Azide |
| Concentration |
1 mg/ml |
| Purity |
Immunogen affinity purified |
Background
HiLyte Fluor(TM) 680-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. The fluorescence of HiLyte Fluor(TM) 680 is only slightly red-shifted compared to those of Cy5.5 dyes, resulting in an optimal match to filters designed for Cy5.5 dyes. Fluorescence emission of HiLyte Fluor(TM) 680 is well separated from that of other commonly used red fluorophores, such as TAMRA, R-phycoerythrin and HiLyte Fluor(TM) 647 dyes, making it ideal for three- and four-color labeling. The fluorescence signal of HiLyte Fluor(TM) 680-conjugate can be observed at 699 nm with the excitation at 678 nm.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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