Maleimide Activated Streptavidin Summary
| Description |
Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance. | Features | Benefits | | Compatible protein thiolation kit available | No specialist knowledge required | | Covalent bond | Highly stable conjugates | | Maleimide activated labels can be used with a range of biomolecules, including oligonucleotides | Flexible | | Scalable | Easy transfer from R&D to manufacturing | | Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility | | Traditional, well-known conjugation chemistry | Trusted method |
If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label: 1. Protein Thiolation Kit Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein. Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working. 2. Thiol Quantification Kit The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction. The assay is in a convenient 96-well plate format and contains all the necessary reagents required. Molecules that do not contain -SH groups can be chemically modified to introduce the required thiol functionality. In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. |
| Specificity |
The reactive maleimide group that has been added to the Streptavidin allows conjugation reactions to be carried out in the pH range 6.5-7.5, creating a stable thio-ether link. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
|
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
Supplied as freeze dried formulations |
| Preservative |
No Preservative |
Notes
This product is manufactured by Abcam and distributed by Novus Biologicals.
Background
Maleimide activated proteins react readily with molecules that contain sulfhydryl (thiol) groups to create a stable thioether linkage. Innova's activated proteins are supplied as a stable freeze dried formulation; all materials are rigorously QC tested using thiolated biomolecules to ensure high efficiency conjugation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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