MTH1 Antibody Summary
||Human, Mouse, Rat
||WB, ICC/IF, IHC, IHC-Fr, IHC-P
Immunogen affinity purified
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MTH1 Antibody Details
A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]
Human, Mouse and Rat.
||Read Publications using NB100-109 in the following species:
- Western Blot 1:500
- Immunocytochemistry/Immunofluorescence 1:50-1:200
- Immunohistochemistry 1:200
- Immunohistochemistry-Frozen 1:200
This MTH1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB, a band can be seen at ~18kDa.
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MTH1 Antibody (NB100-109) has been mentioned in at least 8 publications
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Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for MTH1 Antibody
- 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase
- EC 3.6.1.-
- MTH1Nucleoside diphosphate-linked moiety X motif 1
- mutT human homolog 1,8-oxo-dGTPase
- nucleoside diphosphate-linked moiety X-type motif 17,8-dihydro-8-oxoguanine triphosphatase
Related Products by Gene
MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.
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Publications for MTH1 Antibody (NB100-109) (8)
Read what people are writing who have used MTH1 Antibody (NB100-109).
We have publications tested in 3 confirmed species: Human, Mouse, Rat.
We have publications tested in 6 applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P.
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|Rai P, Young JJ, Burton DG et al. Enhanced elimination of oxidized guanine nucleotides inhibits oncogenic RAS-induced DNA damage and premature senescence. Oncogene. 2010 Nov 15. [PMID: 21076467] (IB, Human)
|Takeshita, F et al. Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine. Cancer Lett;194(1):25-35. 2003 May 8. [PMID: 12706856] (WB, IHC-P, Rat)
|Kennedy, CH et al. Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue. Free Radic Biol Med;34(11):1447-57. 2003 Jun 1. [PMID: 12757855] (WB, IHC-Fr, Human)
|Rai P et al. Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. Proc Natl Acad Sci U S A;106(1):169-74. 2009 Jan 6. [PMID: 19118192]
|Chang IY, Kim JH, Cho KW, Yoon SP. Acute responses of DNA repair proteins and StarD6 in rat hippocampus after domoic acid-induced excitotoxicity Acta Histochem 2012 Aug 7 [PMID: 22883302] (WB, Mouse)
|Sheng Z, Oka S, Tsuchimoto D et al. 8-Oxoguanine causes neurodegeneration during MUTYH-mediated DNA base excision repair J Clin Invest 2012 Dec 3 [PMID: 23143307] (IHC, ICC/IF, Mouse)
|Huber KV, Salah E, Radic B et al. Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy. Nature 2014 Apr 10 [PMID: 24695225] (WB, Human)
|Patel A, Burton DG, Halvorsen K et al. MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene 2014 Jul 14 [PMID: 25023700] (WB, Human)
MTH1 antibody used for WB and IHC-P in human species. In WB, the antibody used on lysates of A549, H358, H23 cells treated or not with lentiviral MTH1 shRNA, and several other experiments involving these cell lines. In IHC-P, the antibody used at 1:150 dilution on xenografts created from the transfected cell lines.
Product General Protocols
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FAQs for MTH1 (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for MTH1
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