MTH1 Antibody 0.2 ml

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Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MTH1 (NB100-109) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - Detection of MTH1 (Green) in Hela cells using NB100-109. Nuclei (Blue) are counterstained with Hoechst 33258.
Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NB100-109

  • Catalog Number
    NB100-109
  • Displayed Format
    Unconjugated
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MTH1 Antibody Summary

Species Human, Mouse, Rat
Tested Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
NUDT1
Purity
Immunogen affinity purified
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MTH1 Antibody Details

Immunogen
A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]

Species Reactivity


Publications Read Publications using NB100-109 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin
Application Notes
This MTH1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB, a band can be seen at ~18kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Molecular Weight
18 kDa
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-109AF405
Alexa Fluor (R) 488 Labeled NB100-109AF488
Alexa Fluor (R) 647 Labeled NB100-109AF647
Alexa Fluor (R) 700 Labeled NB100-109AF700
Biotin Labeled NB100-109B
DyLight 405 Labeled NB100-109V
DyLight 488 Labeled NB100-109G
DyLight 550 Labeled NB100-109R
DyLight 650 Labeled NB100-109C
FITC Labeled NB100-109F
HRP Labeled NB100-109H
Publications
Read Publications using
NB100-109 in the following applications:

Contact Information

Product PDFs

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Reviews

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Publications

MTH1 Antibody (NB100-109) has been mentioned in at least 12 publications.
Earn rewards if you have published using MTH1 Antibody (NB100-109).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Unit Size
0.2 ml
Concentration
1.0 mg/ml
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol NUDT1
Entrez
Uniprot

Alternate Names for MTH1 Antibody

  • 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase
  • EC 3.6.1.-
  • MTH1Nucleoside diphosphate-linked moiety X motif 1
  • mutT human homolog 1,8-oxo-dGTPase
  • nucleoside diphosphate-linked moiety X-type motif 17,8-dihydro-8-oxoguanine triphosphatase
  • nudix (nucleoside diphosphate linked moiety X)-type motif 1,8-oxo-7,8-dihydroguanosine triphosphatase
  • Nudix motif 1
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Related Products by Gene

Background

MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.

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Publications for MTH1 Antibody (NB100-109) (12)

Read what people are writing who have used MTH1 Antibody (NB100-109).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P.

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Filter By Application
WB
(6)
IB
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Rat
(1)
All Species
Publication Applications Species
Rai P, Young JJ, Burton DG et al. Enhanced elimination of oxidized guanine nucleotides inhibits oncogenic RAS-induced DNA damage and premature senescence. Oncogene. 2010 Nov 15. [PMID: 21076467] (IB, Human) IB Human
Takeshita, F et al. Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine. Cancer Lett;194(1):25-35. 2003 May 8. [PMID: 12706856] (WB, IHC-P, Rat) IHC-P, WB Rat
Kennedy, CH et al. Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue. Free Radic Biol Med;34(11):1447-57. 2003 Jun 1. [PMID: 12757855] (WB, IHC-Fr, Human) IHC-Fr, WB Human
Rai P et al. Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. Proc Natl Acad Sci U S A;106(1):169-74. 2009 Jan 6. [PMID: 19118192]
Chang IY, Kim JH, Cho KW, Yoon SP. Acute responses of DNA repair proteins and StarD6 in rat hippocampus after domoic acid-induced excitotoxicity Acta Histochem 2012 Aug 7 [PMID: 22883302] (WB, Mouse) WB Mouse
Sheng Z, Oka S, Tsuchimoto D et al. 8-Oxoguanine causes neurodegeneration during MUTYH-mediated DNA base excision repair J Clin Invest 2012 Dec 3 [PMID: 23143307] (IHC, ICC/IF, Mouse) ICC/IF, IHC Mouse
Huber KV, Salah E, Radic B et al. Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy. Nature 2014 Apr 10 [PMID: 24695225] (WB, Human) WB Human
Patel A, Burton DG, Halvorsen K et al. MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene 2014 Jul 14 [PMID: 25023700] (WB, Human)

Details:
MTH1 antibody used for WB and IHC-P in human species. In WB, the antibody used on lysates of A549, H358, H23 cells treated or not with lentiviral MTH1 shRNA, and several other experiments involving these cell lines. In IHC-P, the antibody used at 1:150 dilution on xenografts created from the transfected cell lines.
WB Human
Bruel L, Sulzenbacher G, Cervera Tison M et al. alpha-Galactosidase/sucrose kinase (AgaSK), a novel bifunctional enzyme from the human microbiome coupling galactosidase and kinase activities. J Biol Chem 2011 Nov 25 [PMID: 21931163]
Carter M, Jemth AS, Hagenkort A et al. Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2. Nat Commun. 2015 Aug 04 [PMID: 26238318] (WB, Human)

Details:
MTH1 antibody used for WB on U2OS cells that were transfected with MTH1 siRNA for 96 hours (Figure 4a). The primary antibody was used at a dilution of 1:1,000 with overnight incubation at 4C.
WB Human
Giribaldi MG, Munoz A, Halvorsen K et al. MTH1 expression is required for effective transformation by oncogenic HRAS. Oncotarget 2015 May 10 [PMID: 25893378] (Human) Human
Huber KV, Olek KM, Muller AC et al. Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling. Nat Methods 2015 Nov [PMID: 26389571]

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics Tool for MTH1

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Research Areas for MTH1

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PTMs for MTH1

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Blogs on MTH1

Check out the latest blog posts on MTH1.
MTH1: Effects on DNA Damage Repair, Cancer and Neurodegeneration
MTH1 (human MutT Homolog 1) is a purine nucleoside triphosphatase enzyme and belongs to the Nudix hydrolase family. In mammalian systems, MTH is a major detoxifier of the oxidized DNA precursors, 8-oxo-dGTP, 8-oxo-dATP, and 2-OH-dATP and prevents the...  Read full blog post.