A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side
Human and mouse.
Western Blot 1:2000
Simple Western 1:40
This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommendedIn Simple Western only 10-15 uL of the recommended dilution is used per data point.
Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.
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FAQs for MP1/MAP2K1IP1 (2)
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)