MAP2K1IP1/MAPKSP1 Antibody 0.025 ml

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Western Blot: MAP2K1IP1/MAPKSP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Immunocytochemistry/Immunofluorescence: MAP2K1IP1/MAPKSP1 Antibody [NBP1-50631] - ICC staining of MAP2K1IP1 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MAP2K1IP1/MAPKSP1 Antibody [NBP1-50631] - IHC staining of MAP2K1IP1 in mouse lung.
Simple Western: MAP2K1IP1/MAPKSP1 Antibody [NBP1-50631] - Simple Western lane view shows a specific band for MP1/MAP2K1IP1 in 0.5 mg/ml of A431 lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NBP1-50631

  • Catalog Number
    NBP1-50631
  • Displayed Format
    Unconjugated
  • Other Formats
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MAP2K1IP1/MAPKSP1 Antibody Summary

Species Human, Mouse
Tested Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
LAMTOR3
Purity
Immunogen affinity purified
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MAP2K1IP1/MAPKSP1 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side

Species Reactivity

Human and mouse.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:40
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommendedIn Simple Western only 10-15 uL of the recommended dilution is used per data point.
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-50631AF405
Alexa Fluor (R) 488 Labeled NBP1-50631AF488
Alexa Fluor (R) 647 Labeled NBP1-50631AF647
Alexa Fluor (R) 700 Labeled NBP1-50631AF700
Biotin Labeled NBP1-50631B
DyLight 405 Labeled NBP1-50631V
DyLight 488 Labeled NBP1-50631G
DyLight 550 Labeled NBP1-50631R
DyLight 650 Labeled NBP1-50631C
FITC Labeled NBP1-50631F
HRP Labeled NBP1-50631H

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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Unit Size
0.025 ml
Concentration
1.08 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol LAMTOR3
Entrez
Uniprot

Alternate Names for MAP2K1IP1/MAPKSP1 Antibody

  • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3
  • late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
  • MAP2K1IP1
  • MAPBP
  • MAPK scaffold protein 1
  • MAPKSP1
  • MEK binding partner 1
  • MEK partner 1
  • MEK-binding partner 1
  • mitogen-activated protein kinase kinase 1 interacting protein 1
  • Mitogen-activated protein kinase kinase 1-interacting protein 1
  • Mitogen-activated protein kinase scaffold protein 1
  • Mp1
  • MP1
  • PRO0633
  • Ragulator3
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Related Products by Gene

Background

Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.

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Product General Protocols

View specific protocols for MP1/MAP2K1IP1 Antibody (NBP1-50631): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for MP1/MAP2K1IP1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for MP1/MAP2K1IP1

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