MDM1 Antibody 0.1 ml

Click image to view larger
Western Blot: MDM1 Antibody [NBP1-85839] - WB for MDM1 in 293T cells. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MDM1 Antibody [NBP1-85839] - Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & centrosome.
Immunohistochemistry-Paraffin: MDM1 Antibody [NBP1-85839] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Western Blot: MDM1 Antibody [NBP1-85839] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp
Download Datasheet
See All Related

Ordering Information: NBP1-85839

  • Catalog Number
    NBP1-85839
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 ml
  • Availability
  • Price
    Shipping
  

MDM1 Antibody Summary

Species Human
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
MDM1
Purity
Immunogen affinity purified
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

MDM1 Antibody Details

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:IPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKEN

Species Reactivity

Human
Reviewed Species Read 1 rated 4
using NBP1-85839 in the following species:

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended.
Reviewed Applications
Read 1 rated 4
using
NBP1-85839 in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 4
Recent Reviews
WB Tested In Human
Reviewed On: 07/02/2014
Rated: 4

MDM1 NBP1-85839
Image 1 of 1
Earn rewards points by submitting your review for MDM1 Antibody (NBP1-85839)

Publications

Earn rewards if you have published using MDM1 Antibody (NBP1-85839).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Unit Size
0.1 ml
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol MDM1

Alternate Names for MDM1 Antibody

  • FLJ95264
  • Mdm1 nuclear protein homolog (mouse)
  • Mdm4, transformed 3T3 cell double minute 1, p53 binding protein
  • Mdm4, transformed 3T3 cell double minute 1, p53 binding protein (mouse)
  • nuclear protein double minute 1
  • nuclear protein MDM1
Show More

Related Products by Gene


Reviews for MDM1 Antibody (NBP1-85839) (1) 41

Read what people are saying who have used MDM1 Antibody (NBP1-85839).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 4
(Based on 1 reviews)

Have you used MDM1 Antibody (NBP1-85839)? Submit your review and earn rewards points which can be used for merchandise & discounts.

Sort 1 reviews for MDM1 Antibody (NBP1-85839) by:
Filter by Ratings
Very Good
(1)
All Ratings
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Western Blot MDM1 NBP1-85839
Click to Enlarge
Very Good
WB Human Anonymous 07/02/2014
Show

Summary

ApplicationWestern Blot
Sample Tested293T lysates
Sample Pretreatment30 ug SDS lysate
SpeciesHuman

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Publications for MDM1 Antibody (NBP1-85839) (0)

There are no publications for MDM1 Antibody (NBP1-85839).
By submitting a publication earn 200 points for your submission points towards our Rewards Program.
Have you published using MDM1 Antibody (NBP1-85839)? Earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for MDM1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for MDM1

Discover related pathways, diseases and genes to MDM1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

PTMs for MDM1

Learn more about PTMs related to MDM1.

Blogs on MDM1

There are no specific blogs for MDM1, but you can read our latest blog posts.