MDM1 Antibody Summary
WB, ICC/IF, IHC, IHC-P
Immunogen affinity purified
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
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MDM1 Antibody Details
This antibody was developed against Recombinant Protein corresponding to amino acids:IPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKEN
1 rated 4
using NBP1-85839 in the following species:
Western Blot 1:100-1:500 Immunocytochemistry/Immunofluorescence Immunohistochemistry 1:10-1:500 Immunohistochemistry-Paraffin 1:50-1:200
For IHC-Paraffin HIER pH6 retrieval is recommended.
1 rated 4
NBP1-85839 in the following applications:
Frequently Purchased Together
WB Tested In Human
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Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, pH 7.2, containing 40% glycerol
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
0.02% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
Alternate Names for MDM1 Antibody
FLJ95264 Mdm1 nuclear protein homolog (mouse) Mdm4, transformed 3T3 cell double minute 1, p53 binding protein Mdm4, transformed 3T3 cell double minute 1, p53 binding protein (mouse) nuclear protein double minute 1 Show More Related Products by Gene
MDM1 Antibody (NBP1-85839) (1)
Read what people are saying who have used MDM1 Antibody (NBP1-85839).
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 4
(Based on 1 reviews)
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Application Western Blot Sample Tested 293T lysates Sample Pretreatment 30 ug SDS lysate Species Human Blocking
Blocking Details 5% BSA in PBS Primary Anitbody
Dilution Ratio 1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker Secondary Antibody
Secondary Manufacturer Cat# 111-005-045 Secondary Concentration 1:20000 Details
Detection Notes Probed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure
Publications for MDM1 Antibody (NBP1-85839) (0)
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Product General Protocols
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FAQs for MDM1 (3)
I used an Ab NBP-1 85839 from your company in NSLC cells for western and am wondering what the expected size of this protein should be.
The MDM1 protein has an expected molecular weight of 80kDa. There are four isoforms to this protein and our product NBP1-85839 is capable of detecting isoforms 1 and 4 (80 and 76kDa respectively).
uniprot site In western blot, our lab has detected bands from 70-78kDa. Since there is a phophoserine residue at amino acid 83, it is possible to detect bands up to 80-85kDa. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit:
IHC-P Protocol and IHC-P Troubleshooting. I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for MDM1
Discover related pathways, diseases and genes to MDM1. Need help? Read the
Bioinformatics Tool Guide
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