Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This antibody is useful in Immunohistochemistry and Western Blot.
Dilutions:
Western Blot 1/2000,
Immunohistochemistry-Paraffin 1/100
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
PBS
Preservative:
0.1% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Rat MCP-1/JE (monocyte chemoattracctant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library1. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3'-end. This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25 kDa)2. In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli3.
Yoshimura,T. et al. (1991) Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) its expression in rat spleen cells tumor cell lines. Biochem. Biophys. Res. Commun. 174:504-509