MCP1 Antibody (NB100-78196)

MCP1 Antibody (2H5)

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MCP1 Antibody (2H5) Summary:
Species:Hu, Mu, Rt
Applications:ELISA, IF
Clonality:Monoclonal
Gene:CCL2
Purity:Immunogen affinity purified
Host:Armenian Hamster
Specificity:MCP-1 (2H5)
Publications:Antibody has at least 3 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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MCP1 Antibody (2H5) Details:
Clone:2H5
Isotype:IgG
Immunogen:CHO-expressed, recombinant mouse MCP-1
Species Reactivity:

Mouse, Rat, Human.

Applications:
Uses:For ELISA capture applications, a concentration range of 5-10 mug/ml is recommended. For immunofluorescent staining, the suggested use of this reagent is:0.5 mug per one million cells in 100 mul volume. Immunohistochemistry-paraffin/frozen. It is recommended that the reagent be titrated for optimal performance for each application.
Dilutions:ELISA 1:50-1:100, Immunofluorescence 0.5 μg per 106 cells in 100 μl volume
Unit Size:0.5 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:Phosphate-buffered solution, pH 7.2
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CCL2
Entrez6347 (Human)
20296 (Mouse)
24770 (Rat)
 
Background:

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

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Working with MCP1

MCP1 Antibody (2H5) Images (2)
The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. NB100-78198)The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. NB100-78198)
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Publications (3)

MCP1 Antibody (2H5) Background References:

  1. Charo, I., et al., 199
  2. Bischoff, S., et al., 1992. J. Exp. Med. 175:1271.

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RNAi (1)
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Secondary Antibodies
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MCP1 in Context


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