Lipase A Antibody 0.025 ml

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Western Blot: Lipase A Antibody [NBP1-54155] - WB detection of LIPA in HepG2 whole cell extract.
Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP1-54155] - ICC staining of LIPA in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: Lipase A Antibody [NBP1-54155] - IHC staining of LIPA in mouse stomach smooth muscle.
Simple Western: Lipase A Antibody [NBP1-54155] - Simple Western lane view shows a specific band for Lipase A in 0.5 mg/ml of HepG2 lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NBP1-54155

  • Catalog Number
    NBP1-54155
  • Displayed Format
    Unconjugated
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Lipase A Antibody Summary

Species Human, Mouse
Tested Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
LIPA
Purity
Immunogen affinity purified
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Lipase A Antibody Details

Immunogen
A genomic peptide made to an internal region of the human Lipase A protein (within residues 150-300). [Swiss-Prot P38571]
Localization
Lysosome.

Species Reactivity

Human and mouse. Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.
Publications Read Publications using NBP1-54155 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:10000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This LIPA antibody is useful for ICC/IF, IHC-P and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Positive Controls
Publications
Read a Publication using
NBP1-54155 in the following applications:

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Publications

Lipase A Antibody (NBP1-54155) has been mentioned in at least 1 publication.
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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, 50% glycerol
Unit Size
0.025 ml
Concentration
0.67 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol LIPA
Entrez
Uniprot

Alternate Names for Lipase A Antibody

  • Acid cholesteryl ester hydrolase
  • Cholesteryl esterase
  • EC 3.1.1
  • EC 3.1.1.13
  • LALCESDcholesterol ester hydrolase
  • Lipase A
  • lipase A, lysosomal acid, cholesterol esterase
  • lysosomal acid lipase
  • lysosomal acid lipase/cholesteryl ester hydrolase
  • Sterol esterase
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Related Products by Gene

Background

Lipase A (LIPA) is an enzyme essential for biotransformation, sequestration and breakdown of cholesterol. It is localized in lysosomes and is expressed at high levels in small intestinal villi, the zona fasciculate/reticularis of the adrenal cortex, pancreatic acini, renal tubular epithelium, hepatocytes, spleenocytes and thymic cells. LIPA is highly crucial for hydrolysis of cholesteryl esters and triglycerides internalized via receptor-mediated endocytosis of lipoproteins. It is also implicated in mediating LDL uptake effects on suppression of HMG-CoA reductase and activation of endogenous cholesteryl ester synthesis. Defective LIPA in liver and kidney may lead to lipoprotein cholesterol accumulation which results in pathologies including atherosclerosis, inflammation, NIDDM and chronic kidney disease. Defects in LIPA are the cause of Wolman disease (WOD) and cholesteryl ester storage disease (CESD).

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Publications for Lipase A Antibody (NBP1-54155) (1)

Read what people are writing who have used Lipase A Antibody (NBP1-54155).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: WB, ICC/IF.

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Product General Protocols

View specific protocols for Lipase A Antibody (NBP1-54155): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Lipase A (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for Lipase A

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Research Areas for Lipase A

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PTMs for Lipase A

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Blogs on Lipase A

Check out the latest blog posts on Lipase A.
How do Lipase A and the CD36 Antibody Relate to Each Other
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