Lipase A Antibody Summary
||WB, Simple Western, ICC/IF, IHC, IHC-P
Immunogen affinity purified
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Lipase A Antibody Details
A genomic peptide made to an internal region of the human Lipase A protein (within residues 150-300). [Swiss-Prot P38571]
Human and mouse. Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.
||Read Publications using NBP1-54155 in the following species:
- Western Blot 1:10000
- Simple Western 1:10000
- Immunocytochemistry/Immunofluorescence 1:50-1:100
- Immunohistochemistry 1:100
- Immunohistochemistry-Paraffin 1:100
This LIPA antibody is useful for ICC/IF, IHC-P and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
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Lipase A Antibody (NBP1-54155) has been mentioned in at least 1 publication
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Manufactured by Genomic Antibody Technology™. GAT FAQs
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, 50% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for Lipase A Antibody
- Acid cholesteryl ester hydrolase
- Cholesteryl esterase
- EC 3.1.1
- EC 184.108.40.206
- LALCESDcholesterol ester hydrolase
Related Products by Gene
, BILE SALT-STIMULATED LIPASE
, PANCREATIC CHOLESTEROL ESTERASE
Lipase A (LIPA) is an enzyme essential for biotransformation, sequestration and breakdown of cholesterol. It is localized in lysosomes and is expressed at high levels in small intestinal villi, the zona fasciculate/reticularis of the adrenal cortex, pancreatic acini, renal tubular epithelium, hepatocytes, spleenocytes and thymic cells. LIPA is highly crucial for hydrolysis of cholesteryl esters and triglycerides internalized via receptor-mediated endocytosis of lipoproteins. It is also implicated in mediating LDL uptake effects on suppression of HMG-CoA reductase and activation of endogenous cholesteryl ester synthesis. Defective LIPA in liver and kidney may lead to lipoprotein cholesterol accumulation which results in pathologies including atherosclerosis, inflammation, NIDDM and chronic kidney disease. Defects in LIPA are the cause of Wolman disease (WOD) and cholesteryl ester storage disease (CESD).
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Publications for Lipase A Antibody (NBP1-54155) (1)
Read what people are writing who have used Lipase A Antibody (NBP1-54155).
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 2 applications: WB, ICC/IF.
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FAQs for Lipase A (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
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