Lightning-Link B-Phycoerythrin Antibody Labeling Kit 1 x 100ug Reaction



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Ordering Information: 716-0005

  • Catalog Number
    716-0005
  • Size(s)
    1 x 100ug Reaction
  • Availability
  • Price
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Lightning-Link B-Phycoerythrin Antibody Labeling Kit Summary

Specificity
Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.

The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.

Our Antibody Concentration and Clean Up kits are a user friendly tool that allows quick and easy concentration of antibodies and proteins. Visit this link to read more about our Antibody Concentration and Clean Up Products.
Kit Type
Antibody Labeling Kit
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.

Lightning-Link B-Phycoerythrin Antibody Labeling Kit Details

Format
B-Phycoerythrin (A=545;563, E=578)

Applications/Dilutions

Publications
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716-0005 in the following applications:

Contact Information

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Reviews

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Publications

Lightning-Link B-Phycoerythrin Kit (716-0005) has been mentioned in at least 6 publications.
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Notes

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
Lightning Link™ is a registered trademark of Innova Biosciences.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Unit Size
1 x 100ug Reaction
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Background

Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

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Publications for Lightning-Link B-Phycoerythrin Kit (716-0005) (6)

Read what people are writing who have used Lightning-Link B-Phycoerythrin Kit (716-0005).

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Product General Protocols

View specific protocols for Lightning-Link B-Phycoerythrin Kit (716-0005): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for Lightning-Link B-Phycoerythrin (4)

  1. What functional groups do I need on my protein?
    • Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.
  2. Can non-antibody molecules be labeled?
    • Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.
  3. Do I need to purify the conjugate?
    • No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.
  4. I was wondering if any of the Lightning-Link kit components contain phosphates, and whether you can tell me the concentration?
    • The Lightning-Link kits do contain phosphate. The final concentration will vary depending on the amount of antibody you add to the vial, but should always be less than 15mM.

Additional Lightning-Link B-Phycoerythrin Products

Lightning-Link B-Phycoerythrin 716-0005

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