Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.
The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.
Our Antibody Concentration and Clean Up kits are a user friendly tool that allows quick and easy concentration of antibodies and proteins. Visit this link to read more about our Antibody Concentration and Clean Up Products.
Antibody Labeling Kit
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs Lightning Link™ is a registered trademark of Innova Biosciences.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
1 x 100ug Reaction
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
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