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Lightning-Link B-PE Antibody Labeling Kit 1x 100ug Reaction

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Catalog Number  716-0005
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  • Size(s)1x 100ug Reaction
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Jump to Information on Lightning-Link B-PE Antibody Labeling Kit (716-0005)

Lightning-Link B-PE Antibody Labeling Kit Summary
 
Specificity Lightning-Link is the easiest and quickest method available for making antibody (protein) conjugates, requiring just 30 seconds hands-on time. The conjugation is directional and covalent in a gentle and controlled process at near-neutral pH.B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575.

The amount of antibody used for labeling should be the same as the pack size of LL-B-PE, with specific information available on our protocol page. For any new antibody, optimization of the ratio of antibody to dye is often worthwhile.

If your antibody needs to be concentrated, purified, or cleaned up, please look at our antibody clean up kits.
Kit Type Antibody Labeling Kit
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Lightning-Link B-PE Antibody Labeling Kit Details
 
Conjugate B-Phycoerythrin (A=545;563, E=578)
Applications/Dilutions
 
Application Notes

This kit contains 1x100ug reactions
Directly Conjugated
Formats
B-Phycoerythrin Labeled 716-0015
B-Phycoerythrin Labeled 716-0010
Learn about Custom Antibody Labeling
Publications
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716-0005 in the following applications:
Product PDFs
  Download PDF Datasheet
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  Download Protocol 1
  Download Protocol 2
  Request MSDS
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Publications
Lightning-Link B-PE Antibody Labeling Kit (716-0005) has been mentioned in at least 6 publications.
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Protocols and FAQs
Controls & Support Products
View all related Lightning-Link B-PE Antibody Labeling Controls and Support Products.
Notes
 
Storage and components

The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20 degrees Celsius upon receipt.

Summary of features/benefits:
Easy
No solvents
No powders to weigh out
No desalting/dialysis
High efficiency
100% recovery
Freely scalable (custom pack sizes if required)

Kit contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size)
1 vial of LL-AP Modifier reagent
1 vial of LL-AP Quencher reagent

Frequently Asked Questions:

Q1. What functional groups do I need on my protein?

Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

Q2. Can non-antibody molecules be labeled?

Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

Q3. Do I need to purify the conjugate?

No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

Learn more about Lightning-Link Conjugation Kits by reading FAQs

Lightning Link is a registered trademark of Innova Biosciences.
Packaging, Storage & Formulations
 
Storage Store at -20C. Avoid freeze-thaw cycles.
Unit Size 1x 100ug Reaction
Concentration Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Background
 
Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.