Storage and components

The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20 degrees Celsius upon receipt.

Summary of features/benefits:
Easy
No solvents
No powders to weigh out
No desalting/dialysis
High efficiency
100% recovery
Freely scalable (custom pack sizes if required)

Kit contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size)
1 vial of LL-AP Modifier reagent
1 vial of LL-AP Quencher reagent

Frequently Asked Questions:

Q1. What functional groups do I need on my protein?

Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

Q2. Can non-antibody molecules be labeled?

Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

Q3. Do I need to purify the conjugate?

No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

Learn more about Lightning-Link Conjugation Kits by reading FAQs

Lightning-Link is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH. Alkaline Phosphatase Alkaline phosphatase (AP) is a hydrolyase enzyme that is frequently conjugated to antibodies for use in immunoassays. PNPP is a popular colorimetric substrate for AP; the product of the reaction, p-nitrophenol, can be read at 405nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate; MUP) may also be employed. Features: - 30 seconds hands-on time - No separation steps - No losses - Scalable technology