Storage and components

The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20 degrees Celsius upon receipt.

Summary of features/benefits:
Easy
No solvents
No powders to weigh out
No desalting/dialysis
High efficiency
100% recovery
Freely scalable (custom pack sizes if required)

Kit contents:
Glass vial(s) of Lightning-LinkTM mix (1 or 3 vials, depending on pack size)
1 vial of LL-AP Modifier reagent
1 vial of LL-AP Quencher reagent

Frequently Asked Questions:

Q1. What functional groups do I need on my protein?

Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

Q2. Can non-antibody molecules be labeled?

Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

Q3. Do I need to purify the conjugate?

No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

Learn more about Lightning-Link Conjugation Kits by reading FAQs

Easy AP labeling of your antibody with 30 seconds hands-on time. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.