LDL Receptor Antibody (NB110-57162)
LDL Receptor Antibody (EP1553Y)
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Distributor Data...
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anti-FH antibody, anti-Low density lipoprotein receptor familial hypercholesterolemia antibody, anti-Low Density Lipoprotein Receptor antibody, anti-LDLR antibody, anti-FHC antibody, |
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LDL Receptor Antibody (EP1553Y) Summary: | | Gene: | LDLR |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | This antibody detects both isoforms of LDL receptor. |
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| Publications: | Antibody has at least 3 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional LDL Receptor Products LDL Receptor Antibody (EP1553Y) Details: | Clone: | EP1553Y |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues on the C-terminus of human LDL receptor was used as an immunogen. |
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Reacts in Human, Mouse, Rat. |
| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 140/160. |
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| Dilutions: | Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:100-250, Immunocytochemistry 1:100-250, Immunoprecipitation 1:50, Flow Cytometry 1:70 |
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| TMW: | 150 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis (1). Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits (2). It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) was shown to be mediated by LDL receptors on cultured cells (3).
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Images (3) Reviews (1) Related Products (31) Exploring LDL Receptor Working with LDL Receptor
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with LDL Receptor
LDL Receptor Antibody (EP1553Y) Images (3) Western Blot: LDL Receptor Antibody (EP1553Y) [NB110-57162] - Western blot analysis on HepG2 cell lysate using anti-LDL receptor, dilution 1:5000. |  Immunohistochemistry: LDL Receptor Antibody (EP1553Y) [NB110-57162] - Immunohistochemical staining of paraffin-embedded human liver. |  Immunofluorescent: LDL Receptor Antibody (EP1553Y) [NB110-57162] - Immunofluorescent staining of HEPG2 cells. |
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Reviewed by:
Anonymous 7/30/2009
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Application:
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Western Blot
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Sample Tested:
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cell lines (Hep2 HepG2)
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Sample Loaded:
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50ug
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Lot:
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YD112703
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Results:
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"So far the best antibody for LDLr receptor. I did try a bunch of them (from Abcam for example without success).
Wish you have one good antibody for the LRP receptor"
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Blocking Buffer:
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TBST 5% milk
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Concentration:
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5
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Blocking Time:
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1 hour
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Blocking Temperature:
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Room Temperature
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Dilution Ratio:
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1/500
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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TBST
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Method:
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hrp
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Exposure Time:
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2 minutes
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Positive Control:
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Liver extract
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Specific Bands:
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150 kDa
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Non-specific Bands:
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no much kDa
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Publications (3)LDL Receptor Antibody (EP1553Y) Background References:- Agnello V, et al. Proc Natl Acad Sci 96(22):12766-71, 1999
- Davis CG, et al. J Biol Chem 262(9):4075-82, 1987.
more... - Brown MS, et al. Proc Natl Acad Sci 76(7):3330-7, 1979.
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Related ProductsPositive ControlsLysates (2)Peptides and ProteinsPrimary Antibodies (24)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| LDL Receptor Antibody (C7) | NBP1-78159 | Bv, Hu | ICC, IF, IHC-P, IP, WB | (2) | (6) | | | | LDL Receptor Antibody | NBP1-06709 | Hu, Mk, Mu | WB | (1) | (3) | | | | LDL Receptor Antibody | NBP1-87476 | Hu | IF, IHC-P, WB | (2) | | | | | LDL Receptor Antibody | NBP1-87579 | Hu | IF, IHC-P, WB | (1) | | | | | LDL Receptor Antibody | NBP1-77029 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ... and 19 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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| LDL Receptor in Context
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Novus Explorer for LDL Receptor gene
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