LC3B Antibody (R-155-100)
LC3B Antibody
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anti-Microtubule-associated proteins 1A/1B light chain 3B antibody, biosensis. GENE NAME: MAP1LC3B antibody, anti-Microtubule-associated protein 1 light chain 3 beta antibody, anti-MAP1ALC3 antibody, anti-MAP1A/MAP1B LC3 B antibody, anti-MAP1A/1B light chain 3 B antibody, anti-MAP1 light chain 3-like protein 2 antibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody, anti-Autophagy-related protein LC3 B antibody, anti-APG8b |
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LC3B Antibody Summary: | | Gene: | MAP1LC3B |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | CAUTION: Ref.3 has shown that the protein is cleaved at Lys-121 but Ref.5 has shown that the cleavage site is at Gly-119 as in other mammalian orthologs. |
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| Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional LC3B Products LC3B Antibody Details: | Immunogen: | A synthetic peptide (FEQRVEDVRLIC) corresponding to the N-terminal of human MAP1LC3 B (MAP1LC3B, APG8b) protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B (MAP1LC3B, APG8b) protein. |
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| Localization: | Accession Number: MLP3B_HUMAN ; MLP3B_MOUSE ; MLP3B_RAT |
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| Marker: | Autophagosomes Marker |
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Human, rat. Other species not yet tested. |
| Uses: | Immunohistochemistry-P, immunofluorescence, and Western Blot. The optimal dilution should be determined by the end user. |
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| Dilutions: | Immunofluorescence 1:100-1:1000, Immunohistochemistry-Paraffin 1:100-1:1000, Western Blot 1:100-1:1000 |
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| TMW: | 14.7 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | lyoph |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | Lyophilized. Add 100 ul diH20 to reconstitute. |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. INTERACTION: Q9H1J2:-; NbExp=1; IntAct=EBI-373144, EBI-372073; SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid-anchor. Note=LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.
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Images (2) Reviews (0) Related Products (18) Exploring LC3B Working with LC3B
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Jump to: Publications, Images, Ask a Question
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Working with LC3B
LC3B Antibody Images (2) Western Blot: MAP1LC3 B Antibody [R-155-100] - Western blot under reduceding condition on Olfactory cell line (Odora) lysate using Rabbit antibody to MAP1LC3 B (MAP1LC3B, APG8b): whole serum (R-155-100) at a dilution of 1:100. |  Western Blot: LC3B Antibody [R-155-100] - Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to MAP1LC3 B (MAP1LC3B, APG8b): whole serum (R-155-100) at a dilution of 1:100. |
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Publications (1)LC3B Antibody Product Specific:- Difilippantonio S, et al. Eur. J. Cancer 39:1936-1947(2003). ;
2. Yang Y.-S, et al. Endocr. Relat. Cancer 10:621-627(2003). ;
3. He H, et al. J. Biol. Chem. 278:29278-29287(2003). ;
4. Ota T, et al. Nat. Genet. 36:40-45(2004). ;
5. Tanida I, et al. J. Biol. Chem. 279:36268-36276(2004). ;
6. Tanida I, et al. J. Biol. Chem. 279:47704-47710(2004). ;
7. Tanida I, et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004). ;
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