LC3B Antibody (NB600-1384)
LC3B Antibody
Working with LC3B
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Publications (8)
Product Specific:
- Aoki H, Kondo Y, Aldape K, et al. Autophagy. Monitoring autophagy in glioblastoma with antibody against isoform B of human microtubule-associated protein 1 light chain 3. 2008 May 16;4(4):467-75. [pubmed: 18259115]
- Pyo, J., Nah, J., Kim, H., et al. Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death. Autophagy. April 2008; 4:3, 315-321.
Images (5)
![Western Blot: LC3B antibody [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.](/sites/default/files/p_39019_1.jpg "Western Blot: LC3B antibody [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.")
Western Blot: LC3B antibody [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384. |
![Immunocytochemistry: LC3B antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.](/sites/default/files/p_39019_2.jpg "Immunocytochemistry: LC3B antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.")
Immunocytochemistry: LC3B antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384. |
![Immunofluorescence: LC3B Antibody [NB600-1384] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.](/sites/default/files/p_39019_3.jpg "Immunofluorescence: LC3B Antibody [NB600-1384] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.")
Immunofluorescence: LC3B Antibody [NB600-1384] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI. |
![Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors using NB600-1384.](/sites/default/files/p_39019_4.jpg "Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors using NB600-1384.")
Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors using NB600-1384. |
![Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue using NB600-1384.](/sites/default/files/p_39019_5.jpg "Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue using NB600-1384.")
Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue using NB600-1384. |
Reviews (2) Have you used this product? Submit a review to earn Novus Rewards.
Maghnus O\'Seaghdha on 8/28/2009
It may be that the LC3 protein is difficult to detect in autophagosomes in Keratinocytes. Not yet tested in HeLa.
| Primary Antibody: | Diluted |
|---|---|
| Dilution | 1/2000 |
| Incubation Time: | 16h |
| Incubation Temp: | 4 |
| Secondary Name: | Molecular Probes |
|---|---|
| Host Species: | Goat |
| Conjugation: | Alexa-Fluor 488 |
| Diluted? | Diluted |
| Dilution: | 1/200 |
| Blocking Buffer: | BSA |
| Blocking Concentration: | 2 |
| Blocking Time: | 1 h |
| Blocking Temp | 22 |
New Sample - This antibody has not been tested on Keratinocytes previously. Customer found no positive signal. Nouvs recommends testing Hela cells for a positive control
Sandra Barth on 5/26/2009
| Primary Antibody: | Diluted |
|---|---|
| Dilution | 1/2000 |
| Incubation Time: | overnight |
| Incubation Temp: | 4 |
| Amount Loaded: | 100 |
| Gel Details: | SDS-gel 15% blot 1:15 hr on PVDF membrane wet method |
| Detection Method | ECL-x-ray |
| Used Controls: | False |
| Observed Bands: | True |
| Specific Bands: | 15 kDa |
| Nonspecific Bands: | 20 kDa, 37 kDa, 82 kDa |
| Secondary Name: | goat-anti rabbit-HRP (Pierce) |
|---|---|
| Host Species: | goat |
| Conjugation: | HRP |
| Diluted? | Diluted |
| Dilution: | 1/2000 |
| Blocking Buffer: | milk/PBS |
| Blocking Concentration: | 5 |
| Blocking Time: | 1 hour |
| Blocking Temp | room temperature |