LC3B Antibody (NB600-1384)
LC3B Antibody
Working with LC3B
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Publications (9)
Product Specific:
- X. Li, C.-E. Wang, S. Huang, et al. Inhibiting the ubiquitin-proteasome system leads to preferential accumulation of toxic N-terminal mutant huntingtin fragments. Hum. Mol. Genet. 2010; ddq127. [PMID: 20354076]
- Aoki H, Kondo Y, Aldape K, et al. Autophagy. Monitoring autophagy in glioblastoma with antibody against isoform B of human microtubule-associated protein 1 light chain 3. 2008 May 16;4(4):467-75. [pubmed: 18259115] (Mouse, Ab Development, WB, ICC/IF, IHC-F)
Images (6)
![LC3B Antibody [NB600-1384)] Western Blot image](/sites/default/files/p_39019_1.jpg "Western Blot: LC3B antibody [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.")
Western Blot: LC3B antibody [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384. |
![LC3B Antibody [NB600-1384)] Immunocytochemistry image](/sites/default/files/p_39019_2.jpg "Immunocytochemistry: LC3B antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.")
Immunocytochemistry: LC3B antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384. |
![LC3B Antibody [NB600-1384)] Immunofluorescence image](/sites/default/files/p_39019_3.jpg "Immunofluorescence: LC3B Antibody [NB600-1384] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.")
Immunofluorescence: LC3B Antibody [NB600-1384] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI. |
![LC3B Antibody [NB600-1384)] Immunohistochemistry image](/sites/default/files/p_39019_4.jpg "Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors using NB600-1384.")
Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors using NB600-1384. |
![LC3B Antibody [NB600-1384)] Immunohistochemistry image](/sites/default/files/p_39019_5.jpg "Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue using NB600-1384.")
Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue using NB600-1384. |
![LC3B Antibody [NB600-1384)] FACS image](/sites/default/files/42098_6.jpg "Flow Cytometry: LC3 Antibody [NB600-1384] FACS staining of NTERA-2 cells using NB600-1384 at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary")
Flow Cytometry: LC3 Antibody [NB600-1384] FACS staining of NTERA-2 cells using NB600-1384 at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary |
Reviews (2) Have you used this product? Review this product to earn Novus Rewards.
Reviewed by: Maghnus O'Seaghdha 08/28/2009
| Application: | IF |
| Sample Tested: | Human Oral Keratinocyte |
| Species: | Goat |
| Lot: | K |
| Results: | It may be that the LC3 protein is difficult to detect in autophagosomes in Keratinocytes. Not yet tested in HeLa. |
Novus - Comments
08/28/2009 - New Sample - This antibody has not been tested on Keratinocytes previously. Customer found no positive signal. Nouvs recommends testing Hela cells for a positive control.
| Blocking Buffer: | BSA |
| Blocking Time: | 1 hr |
| Blocking Temp: | 22 Celsius |
| Dilution Ratio: | 1/2000 |
| Incubation Time: | 16h |
| Incubation Temp: | 4 Celsius |
| Incubation Dilution Buffer: | 2% BSA |
| Description: | Molecular Probes |
| Manufacturer Catalog #: | A-11034 |
| Dilution Ratio: | 1/200 |
Reviewed by: Sandra Barth 05/26/2009
| Application: | Western Blot |
| Sample Tested: | mouse liver tissue |
| Sample Loaded: | 100 |
| Species: | Mouse |
| Lot: | I1 |
| Blocking Buffer: | milk/PBS |
| Blocking Concentration: | 5 |
| Blocking Time: | 1 hour |
| Blocking Temp: | room temperature |
| Dilution Ratio: | 1/2000 |
| Incubation Time: | overnight |
| Incubation Temp: | 4 Celsius |
| Incubation Dilution Buffer: | milk/PBS |
| Description: | goat-anti rabbit-HRP (Pierce) |
| Dilution Ratio: | 1/2000 |
| Detection Method: | ECL-x-ray |
| Exposure Time: | 1 minute |
| Bands: | Yes |
| Specific Bands: | 15 kDa |
| Non Specific Bands: | 20 kDa, 37 kDa, 82 kDa |
