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Clonality:	Polyclonal
Specificity:	This is specific for the B isoform of LC3.
Purity:	Peptide affinity purified
Host:	Rabbit

Immunogen:	A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.
Localization:	LC3 I: cytoplasmic, LC3 II: Intracytoplasmic membrane; lipid anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid anchor.

Entrez:	81631 (Human)
	67443 (Mouse)

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

	Mouse, Human
Reactivity:	This antibody reacts with the human and mouse proteins. Other species have not been tested..
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

	FACS, IHC-Fr, ICC, WB, IF
Uses:	NB600-1384 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells. LC3-I is seen at ~18 kDa and LC3-II at ~16 kDa.
Dilutions:	flow cytometry / FACS analysis 1:200, Immunocytochemistry 1:2000, Immunofluorescence 1:2000, Immunohistochemistry-Frozen 1:2000, Western Blot 1:3000
Conjugated Ab's:	6 conjugated antibodies

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-glycine, 150mM NaCl
Preservative:	0.05% Sodium Azide
Unit Size:	0.1ml (also available: 0.025ml (sample size))
Concentration:	1.47 mg/ml
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

1. X. Li, C.-E. Wang, S. Huang, et al. Inhibiting the ubiquitin-proteasome system leads to preferential accumulation of toxic N-terminal mutant huntingtin fragments. Hum. Mol. Genet. 2010; ddq127. [PMID: 20354076]
2. Aoki H, Kondo Y, Aldape K, et al. Autophagy. Monitoring autophagy in glioblastoma with antibody against isoform B of human microtubule-associated protein 1 light chain 3. 2008 May 16;4(4):467-75. [pubmed: 18259115] (Mouse, Ab Development, WB, ICC/IF, IHC-F)
3. Pyo JO, Nah J, Kim HJ, et al. Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death. Autophagy. 2008 Apr 1;4(3):315-21. [PMID: 18196969]

9 publications available on website

Application: IF on 08/28/2009 Application: Western Blot on 05/26/2009

Images (6)

	Western Blot: LC3B antibody [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.		Immunocytochemistry: LC3B antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.
	Immunofluorescence: LC3B Antibody [NB600-1384] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.		Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in treated U87-MG cultured & subcutaneous tumors using NB600-1384.
	Immunohistochemistry: LC3B antibody [NB600-1384] - LC3B staining in gliobastoma multiform tissue using NB600-1384.		Flow Cytometry: LC3 Antibody [NB600-1384] FACS staining of NTERA-2 cells using NB600-1384 at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary

1 Lysates

2 Peptides and Proteins

8 Primary Antibodies

1 RNAi

123 Secondary Antibodies

MAP1LC3A, MAP1LC3C, ATG7, ATF4, ATG5, ANXA3, BECN1, EIF2AK3, CASP3, PPARG, SQSTM1, ATG3, DDIT3, BCL2, AKT1, MAPK3, EGR1, PRDX2, FBXO32, DCTN4, KHDRBS1, CDK2, MPZL2, ATG4B, HSPA5, NUP62, TXNRD1, ATG10, IGF2BP2, GABARAPL2, GTF2H1, SYT1, TFRC, MTOR, NKX3-1, TRP53, PNLIP, WNK1, SGSM3, TERT

General Protocols

• Protocol specific for LC3B Antibody (NB600-1384)