LC3B Antibody

Catalog#:	NB600-1384	This product is fully covered under guarantee+

Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer:	Tris-glycine, 150mM NaCl
Preservative:	0.05% Sodium Azide

Purity:	peptide affinity purified
Host:	Rb
Clonality:	Polyclonal
Specificity:	This is specific for the B isoform of LC3.
Modification:	No
Immunogen:	A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.

Entrez:	81631 (Human)
	67443 (Mouse)
Related Genes:	81631

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

	Mu, Hu
reactivity:	This antibody reacts with the human and mouse proteins. Other species have not been tested..

	IHC-Fr, ICC, WB, IF
Uses:	NB600-1384 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells. LC3-I is seen at ~18 kDa and LC3-II at ~16 kDa.
Dilutions:	immunofluorescence 1:2000, Western Blot 1:3000, Immunocytochemistry 1:2000, Immunohistochemistry-Frozen 1:2000

Publications:

Aoki H, Kondo Y, Aldape K, et al. Autophagy. Monitoring autophagy in glioblastoma with antibody against isoform B of human microtubule-associated protein 1 light chain 3. 2008 May 16;4(4):467-75. [pubmed: 18259115] Pyo, J., Nah, J., Kim, H., et al. Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death. Autophagy. April 2008; 4:3, 315-321. Gao M, Yeh PY, Lu Y-S, et al. OSU-03012, a Novel Celecoxib Derivative, Induces Reactive Oxygen Species-Related Autophagy in Hepatocellular Carcinoma. Cancer Res 2008;68(22):9348-9357. 8 publications available on website

General Protocols

• Protocol specific for LC3B Antibody (NB600-1384)

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• Autophagy
• Cancer
• Cellular Markers
• Hypoxia