LC3 Antibody (R-146-100) | Novus Biologicals

LC3 Antibody (R-146-100) (0.1 ml)

Additional Products Available View our LC3 products. Call 303-730-1950 for recommended products	Ordering Information:
	Catalog Number	R-146-100
	Size(s)	0.1 ml	Price:
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anti-Autophagy-related ubiquitin-like modifier LC3 A antibody, anti-microtubule-associated proteins 1A/1B light chain 3A antibody, anti-Autophagy-related protein LC3 A antibody, anti-microtubule-associated proteins 1A/1B light chain 3 antibody, anti-LC3A antibody, anti-MAP1A/1B light chain 3 A antibody, anti-LC3 antibody, anti-ATG8E antibody, anti-MAP1BLC3MAP1A/MAP1B light chain 3 A antibody, anti-MAP1ALC3MAP1A/MAP1B LC3 A antibody, anti-microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1 antibody

LC3 Antibody Summary:

Species:	Hu, Mu, Rt	Gene:	MAP1LC3A
Applications:	WB, IF, IHC-P	Purity:	Whole antisera
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	IHC, WB and ELISA confirmed the specificity for MAP1LC3 A (MAP1LC3A, APG8a).
Publications:	R-146-100 has been mentioned in at least 1 Publication

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LC3 Antibody Details:

Immunogen:	A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen.
Localization:	Accession Number: MLP3A_HUMAN ; MLP3A_MOUSE ; MLP3A_RAT
Marker:	Autophagosomes Marker

Species Reactivity:

Human, rat. Predicted cross-reactivity due to antigen sequence homology: Mouse. Other species not yet tested.

Applications:

Uses:	IHC-P, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Dilutions:	Immunofluorescence 1:100-1:1000, Immunohistochemistry-Paraffin 1:100-1:1000, Western Blot 1:100-1:1000
Unit Size:	0.1 ml
Concentration:	Lyoph

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	Lyophilized. Add diH20 to desired concentration. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAP1LC3A

Entrez:	84557 (Human)
	66734 (Mouse)
	362245 (Rat)
Uniprot:	Q9H492 (Human)

Related Genes:

Background:

FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. LC3-II binds to the autophagic membranes. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.

This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

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LC3 Antibody Images (9)

	Western Blot: MAP1LC3 A Antibody [R-146-100] - Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 100 (ECL).

	Immunofluorescent: MAP1LC3 A Antibody [R-146-100] - A. Confocal microscopy on immunofluorescently detected MHC class II in cytospin-isolated human white blood cells.

	Immunofluorescence: MAP1LC3 A Antibody [R-146-100] - B. Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature.

	C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A (MAP1LC3A, APG8a) appearing in red and MHC class II appearing in green. Here, the merged picture is presented.

	Immunofluorescence: MAP1LC3 A Antibody [R-146-100] - Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A (MAP1LC3A, APG8a) appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

	Immunofluorescence: MAP1LC3 A Antibody [R-146-100] - Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

	Immunofluorescence: MAP1LC3 A Antibody [R-146-100] - Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A (MAP1LC3A, APG8a) appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

	Immunofluorescence: MAP1LC3 A Antibody [R-146-100] - Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A (MAP1LC3A, APG8a) appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

	Immunofluorescence: MAP1LC3 A Antibody [R-146-100] - Confocal microscopy on immunofluorescently detected MAP1LC3 A (MAP1LC3A, APG8a) in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A (MAP1LC3A, APG8a): whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.