LC3 Antibody (NBP1-19167) | Novus Biologicals

LC3 Antibody (NBP1-19167) (0.1 ml)

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	Catalog Number	NBP1-19167 (Sample Size Available)
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anti-Autophagy-related ubiquitin-like modifier LC3 A antibody, anti-microtubule-associated proteins 1A/1B light chain 3A antibody, anti-Autophagy-related protein LC3 A antibody, anti-microtubule-associated proteins 1A/1B light chain 3 antibody, anti-LC3A antibody, anti-MAP1A/1B light chain 3 A antibody, anti-LC3 antibody, anti-ATG8E antibody, anti-MAP1BLC3MAP1A/MAP1B light chain 3 A antibody, anti-MAP1ALC3MAP1A/MAP1B LC3 A antibody, anti-microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1 antibody

LC3 Antibody Summary:

Species:	Hu, Mu, Rt, Bv, Ze	Gene:	MAP1LC3A
Applications:	WB, FACS, IHC-P	Purity:	Peptide affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	Although specificity between LC3A and LC3B has not been tested, this antibody was created to a peptide that has 100% identity to LC3A and 62% identity to LC3B.
Publications:	NBP1-19167 has been mentioned in at least 3 Publications

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LC3 Antibody Details:

Immunogen:	Genomic sequence made to a N-terminal portion of the human LC3A protein [Swiss-Prot# Q9H492].
Localization:	Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle autophagosome membrane; Lipid-anchor. Note= LC3-II binds to the autophagic membranes.
Marker:	Autophagosomes Marker

Species Reactivity:

Human, mouse, bovine, rat and zebrafish. Immunogen has 100% homology to rat and 97% homology to bovine. 94% sequence identity with Zebrafish protein.

Applications:

Uses:	This antibody is useful for immunohistochemistry, and Western blot, where a band is seen ~14 kDa. Immunohistochemistry was done on formalin fixed paraffin embedded sections.
Dilutions:	Flow Cytometry 1:100, Immunohistochemistry-Paraffin 1:100, Western Blot 1:2500
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	0.2 mg/ml
Positive Controls:	2 Positive Controls

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	PBS, 0.1% BSA and 50% glycerol
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: MAP1LC3A

Entrez:	84557 (Human)
	66734 (Mouse)
	64862 (Rat)
Uniprot:	Q9H492 (Human)

Related Genes:

Background:

LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.

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