LC3 Antibody (NB100-2220C)
LC3 Antibody [DyLight 650]
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anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody, anti-Autophagy related protein LC3 A antibody, anti-Microtubule associated proteins 1A/1B light chain 3A antibody, anti-Microtubule associated proteins 1A/1B light chain 3 antibody, anti-Microtubule associated protein 1 light chain 3 alpha antibody, anti-MAP1BLC3 antibody, anti-MAP1ALC3 antibody, anti-MAP1A/MAP1B LC3 A antibody, anti-MAP1A/b light chain 3 B antibody, anti-MAP1A/1B light chain 3 A antibody, anti-MAP1 light chain 3-like protein 2 antibody, anti-MAP1 light chain 3 like protein 1 antibody, anti-LC3A antibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody, anti-Autophagy-related ubiquitin-like modifier LC3 A antibody, anti-Autophagy-related protein LC3 B antibody, anti-Autophagy related ubiquitin like modifier LC3 A antibody, |
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LC3 Antibody [DyLight 650] Summary: | | Gene: | MAP1LC3A |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This is specific for LC3. |
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| Publications: | Antibody has been mentioned in at least 19 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional LC3 Products LC3 Antibody [DyLight 650] Details: | Conjugate: | DyLight 650 |
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| Immunogen: | A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). |
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| Localization: | LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes. |
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| Marker: | Autophagosomes Marker |
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This antibody reacts with human and mouse LC3 protein, though the mouse detection is weaker than the human. No other species have been tested. The immunogen used for this antibody production has 100% homology with rat and Zebrafish, 92% with mouse, 91% with cow, and 84% with Xenopus proteins. |
| Uses: | By Western blot bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. Please note general reference (1) below for ATG5 control lysates used in our image. |
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| Dilutions: | immunohistochemistry 1:200-1:400,
immunoprecipitation 20ug/500ug of protein,
Western Blot 2 ug/ml,
Immunohistochemistry-Paraffin ,
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| Unit Size: | 0.1 ml |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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| Conjugated Antibodies: | 4 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | 50mM sodium borate |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
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Reviews (0) Related Products (40) Exploring LC3 Working with LC3
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Jump to: Publications, Ask a Question
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Working with LC3
LC3 Antibody [DyLight 650] Images (0)
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Publications (19)LC3 Antibody [DyLight 650] Product Specific:- Shen S, Zhang P, Lovchik MA, et al. Cyclodepsipeptide toxin promotes the degradation of Hsp90 client proteins through chaperone-mediated autophagy. J Cell Biol 2009;185(4):629-639. [pubmed: 19433452]
- Autophagy. 2009 Apr;5(3):307-13. Epub 2009 Apr 19. [pubmed:19182529]
more... - Zhu Y, Vergote D, Pardo C, et al. CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy. FASEB J 2009:fj.08-128819 [pubmed: 19380511]
- Huang J, Canadien V, Lam GY, et al. Activation of antibacterial autophagy by NADPH oxidases. PNAS 2009;106(15):6226-6231 [pubmed: 19339495]
- Pacheco CD, Elrick MJ, Lieberman AP. Tau deletion exacerbates the phenotype of Niemann-Pick type C mice implicates autophagy in pathogenesis. Hum Mol Genet 2009;18(5):956-965 [pubmed: 19074461]
- Tang D, Kang R, Xiao W, et al. Quercetin Prevents Lipopolysaccharide-induced HMGB1 Release Proinflammatory Function. Am J Respir Cell Mol Biol 2009:2008-0119OC [pubmed: 19265175]
- Panyasrivanit M, Khakpoor A, Wikan N, et al. Co-localization of constituents of the dengue virus translation replication machinery with amphisomes. J Gen Virol. 2009 Feb;90(Pt 2):448-56. [pubmed: 19141455]
- Sarkar S, Korolchuk V, Renna M. et. al. Methodological considerations for assessing autophagy modulators: a study with calcium phosphate precipitates.
- Yuan H, Perry CN, Huang C, et al. LPS-Induced Autophagy Is Mediated by Oxidative Signaling in Cardiomyocytes is Associated with Cytoprotection. Am J Physiol Heart Circ Physiol 2008:01051.02008.
- Humbey O, Pimkina J, Zilfou JT, et al. The ARF Tumor Suppressor Can Promote the Progression of Some Tumors. Cancer Res 2008;68(23):9608-9613.
- Young JE, Martinez RA, La Spada AR. Nutrient Deprivation Induces Neuronal Autophagy Implicates Reduced Insulin Signaling in Neuroprotective Autophagy Activation. J Biol Chem 2009;284(4):2363-2373.
- Cadwell K, Liu JY, Brown SL, et al. A key role for autophagy the autophagy gene Atg16l1 in mouse human intestinal Paneth cells. Nature 2008;456(7219):259-263.
- Degtyarev M, De Maziere A, Orr C, et al. Akt inhibition promotes autophagy sensitizes PTEN-null tumors to lysosomotropic agents. J Cell Biol 2008;183(1):101-116
- Wong ESP, Tan JMM, Soong W-E, et al. Autophagy-mediated clearance of aggresomes is not a universal phenomenon. Hum. Mol. Genetics. 2008;17(16):2570-2582.
- Burdette DL, Yarbrough ML, Orvedahl A, et al. Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, then cell lysis. PNAS 2008;105(34):12497-12502.
- Miller, B., Zhao, Z., Stephenson, L., et al. The autophagy gene ATG5 plays an essential role in B lymphocyte development. Autophagy. April 2008; 4:3, 309-314.
- Zhang, H., M. Bosch-Marce, et al. Mitochondrial autophagy is a HIF-1-dependent adaptive metabolic response to hypoxia. J. Biol. Chem. April 18 2008; 293(16):10892-10903. (Western Blot)
- Settembre C, Fraldi A, Jahreiss L, et al. A block of autophagy in lysosomal storage disorders. Hum Mol Genet. 2008 January 1, 2008;17(1):119-29.
- Sarkar, S., Krishna G., Imarisio, S., et al. A rational mechanism for combination treatment of Huntington's disease using lithium rapamycin. Hum. Mol. Genet. Jan. 2008; 17: 170-178.
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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| LC3 Antibody [DyLight 488] | NB100-2220G | Hu, Mu, Rt | IHC, IHC-P, IP, WB | | (19) | | | | LC3 Antibody [DyLight 550] | NB100-2220R | Hu, Mu, Rt | IHC, IHC-P, IP, WB | | (19) | | | | LC3 Antibody [Biotin] | NB100-2220B | Hu, Mu, Rt | IHC, IHC-P, IP, WB | (2) | (4) | | | | LC3 Antibody [HRP] | NB100-2220H | Hu, Mu, Rt | IHC, IHC-P, IP, WB | (2) | (4) | | |
Positive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies
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Antibody Packs (1)Lysates (3)Peptides and ProteinsPrimary Antibodies (25)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| LC3 Antibody | NB100-2220 | Hu, Mu, Rt | IF, IHC-P, IP, WB | (2) | (48) | | | | LC3 Antibody | NB100-2331 | Bv, Hu, Mu, Rt, Xp, Ze | IHC-P, IP, WB | (1) | (14) | | | | LC3 Antibody | NBP1-19167 | Bv, Hu, Mu, Rt, Ze | FACS, IHC-P, WB | (3) | (1) | | | | LC3 Antibody | R-146-100 | Hu, Rt | IF, IHC-P, WB | (9) | (1) | | | | LC3 Antibody (EP1983Y) | NB110-57179 | Hu, Mu, Rt | FACS, ICC, IHC-P, IP, WB | (3) | (2) | | | | ... and 20 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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