LC3 Antibody (NB100-2220)
LC3 Antibody
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Distributor Data...
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anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody, anti-Autophagy related protein LC3 A antibody, anti-Microtubule associated proteins 1A/1B light chain 3A antibody, anti-Microtubule associated proteins 1A/1B light chain 3 antibody, anti-Microtubule associated protein 1 light chain 3 alpha antibody, anti-MAP1BLC3 antibody, anti-MAP1ALC3 antibody, anti-MAP1A/MAP1B LC3 A antibody, anti-MAP1A/b light chain 3 B antibody, anti-MAP1A/1B light chain 3 A antibody, anti-MAP1 light chain 3-like protein 2 antibody, anti-MAP1 light chain 3 like protein 1 antibody, anti-LC3A antibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody, anti-Autophagy-related ubiquitin-like modifier LC3 A antibody, anti-Autophagy-related protein LC3 B antibody, anti-Autophagy related ubiquitin like modifier LC3 A antibody |
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LC3 Antibody Summary: | | Gene: | MAP1LC3A |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This antibody is specific for LC3B. |
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| Publications: | Antibody has been mentioned in at least 48 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional LC3 Products LC3 Antibody Details: | Immunogen: | A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). |
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| Localization: | LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes. |
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| Marker: | Autophagosomes Marker |
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This antibody reacts with human, rat and mouse LC3 protein, though the mouse detection is weaker than the human. No other species have been tested. The immunogen used for this antibody production has 100% homology with rat and Zebrafish, 92% with mouse, 91% with cow, and 84% with Xenopus proteins. |
| Uses: | By Western blot bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. Please note general reference (1) below for ATG5 control lysates used in our image. |
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| Dilutions: | Immunofluorescence, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunoprecipitation 20ug/500ug of protein, Western Blot 2ug/ml |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 2 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | Tris-glycine, 150mM NaCl |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
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Images (2) Reviews (10) Related Products (40) Exploring LC3 Working with LC3
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with LC3
LC3 Antibody Images (2) Western Blot: LC3 antibody [NB100-2220] - Detection of LC3 in mouse ES cell lysates. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT SW Medical Center. |  Immunohistochemistry: LC3 antibody [NB100-2220] - Staining of human brain, cerebral cortex, neurons with cell processes using NB100-2220. |
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Reviewed by:
Yu-Ting Yen 11/16/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse
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Species:
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Mouse
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Description:
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anti-rabbit HRP
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Reviewed by:
Gill Chang 11/3/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Dilution Ratio:
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1:8000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Reviewed by:
Chi-Hao Chang 10/31/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Sample Pretreated:
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Rapamycin
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Blocking Buffer:
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5% non-fat milk
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Dilution Ratio:
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1:2000
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Incubation Time:
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Overnight
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Description:
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Goat-anti Rabbit
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Reviewed by:
Anonymous 6/15/2009
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Application:
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IP
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Sample Tested:
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IP Sample
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Species:
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Other
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Species Other:
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not listed
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Lysate Amt:
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0.25mg
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Results:
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I used both NB100-2220ss and NB100-2331ss for IP, NB100-2220ss works very well, but not NB100-2331ss.
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Antibody Dilution Ratio:
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20ug/250ug lysate
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Protein Capture:
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Protein A + Protein B
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Protein Reduction:
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RIPA buffer
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Dilution Ratio:
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1:1000
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Incubation Time:
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3 hrs
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Incubation Temperature:
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4 4
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Additional Comments:
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Detected with NB600-1384.
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Reviewed by:
Anonymous 1/9/2012
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Application:
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Western Blot
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Sample Tested:
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H9C2 extract
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Sample Loaded:
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20ug
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Species:
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Rat
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Sample Pretreated:
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Overexpression protein
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Lot:
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A-K
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Blocking Buffer:
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5% Milk in TBST
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Concentration:
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5% Milk in TBST
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Blocking Time:
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1hr rt
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Blocking Temperature:
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RT
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Dilution Ratio:
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1:1000
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Incubation Time:
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4C ,O/N
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Incubation Temperature:
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4C , Celsius
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Incubation Dilution Buffer:
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TBST
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Description:
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Donkey anti Rabbit
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Manufacturer Catalog #:
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na934v ,GE health
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Dilution Ratio:
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NA934V
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Method:
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ECL PLUS
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Exposure Time:
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3s
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Did you observe bands?:
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Yes
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Specific Bands:
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17kd kDa
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Additional Comments:
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The testing results were good. I am pleased with this Antibody.
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Reviewed by:
Hsiu-Hui Chang 12/5/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Sample Pretreated:
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siRNA
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Results:
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Clear
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Dilution Ratio:
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1:5000
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Incubation Time:
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Overnight
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Incubation Temperature:
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4 Celsius
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Reviewed by:
Chou-Jou Chun 11/21/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse
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Species:
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Mouse
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Sample Pretreated:
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Capsaicin
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Results:
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Clear
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Blocking Buffer:
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5% milk
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Blocking Time:
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1 hr.
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Blocking Temperature:
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RT
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Dilution Ratio:
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1:500
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Reviewed by:
Shu-Wen Hsuan 11/21/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Blocking Buffer:
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5% non-fat milk in TBST
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Dilution Ratio:
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1:500
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Incubation Time:
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overnight
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Method:
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Western Lighting plus ECL
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Reviewed by:
Chi-Hao Wu 10/31/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Sample Pretreated:
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None
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Results:
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Clear and significant!
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Blocking Buffer:
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5% milk in TBST
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Dilution Ratio:
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1:1000
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Incubation Time:
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Overnight
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Incubation Temperature:
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4 Celsius
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Reviewed by:
Anonymous 7/21/2010
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Application:
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Western Blot
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Sample Tested:
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Murine Blastocyst Derived Cells
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Sample Loaded:
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30ug
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Species:
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Mouse
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Sample Pretreated:
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none
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Results:
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This particular antibody worked beautifully for WB, with one minor caveat. After repeated usage, the binding of the antibody to LC3-I seems to drop off significantly vs. LC3-II, making quantification of LC3 levels in reference to each isoform significantly more challenging, and borderline impossible due to these fluctuations
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Blocking Buffer:
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5% Milk in TBST
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Blocking Time:
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1 hour
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Blocking Temperature:
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25 Celsius
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Dilution Ratio:
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1:1000
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Incubation Time:
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1 hour
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Incubation Temperature:
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25 Celsius
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Incubation Dilution Buffer:
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1% BSA in TBST
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Description:
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anti-rabbit HRP conjugated
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Dilution Ratio:
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1:5000
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Method:
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Hydrogen Peroxide ECL kit
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Exposure Time:
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5 minutes
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Did you observe bands?:
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Yes
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Specific Bands:
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15 and 17 kDa
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Non-specific Bands:
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none kDa
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Additional Comments:
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Again, was specific, looked good, but lost potency for LC3-I after repeated usage
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Publications (48)LC3 Antibody Product Specific:- Elgendy M, Sheridan C, Brumatti G, Martin SJ. Oncogenic Ras-Induced Expression of Noxa and Beclin-1 Promotes Autophagic Cell Death and Limits Clonogenic Survival. Mol Cell. 2011 Feb 23.[PMID 21353614]
more... - Garbow JR, Doherty JM, Schugar RC, et al. Hepatic steatosis, inflammation, and ER stress in mice maintained long-term on a very low-carbohydrate ketogenic diet.Am J Physiol Gastrointest Liver Physiol. 2011 Jun;300(6):G956-67.[PMID 21454445]
- Fiorito F, Ciarcia R, Granato GE et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induced autophagy in a bovine kidney cell line.Toxicology. 2011 Oct 13.[PMID:22015590]
- Carmignac V, Svensson M, Körner Z, et al. Autophagy is increased in laminin {alpha}2 chain-deficient muscle and its inhibition improves muscle morphology in a mouse model of MDC1A.Hum Mol Genet. 2011 Sep 27.[PMID:21920942]
- Yang D-S, Stavrides P, Mohan PS, et al. Reversal of autophagy dysfunction in the TgCRND8 mouse model of Alzheimer's disease ameliorates amyloid pathologies and memory deficits. Brain. 2011 [PMID: 21186265]
- Xu B, Hua J, Zhang Y, et al. Proliferating cell nuclear antigen (PCNA) regulates primordial follicle assembly by promoting apoptosis of oocytes in fetal and neonatal mouse ovaries. PLoS ONE. 2011. [PMID: 21253613]
- Wang RC, Levine B. Calcipotriol Induces Autophagy in HeLa Cells and Keratinocytes. J Invest Dermatol. 2011. [PMID:21228817]
- Myeku N, Figueiredo-Pereira ME. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: its association with sequestosome 1/P62. J Biol Chem. 2011 May 2.[PMID: 21536669]
- Molitoris JK, McColl KS, Swerdlow S, et al. Glucocorticoid elevation of dexamethasone-induced gene 2 (Dig2/RTP801/REDD1) mediates autophagy in lymphocytes. J Biol Chem. 2011 Jul 6.[PMID 21733849]
- Signorelli P, Avagliano L, Virgili E, et al. Autophagy in term normal human placentas. Placenta. 2011 Jun;32(6):482-485. [PMID 21459442]
- Cotter DG, d'Avignon DA, Wentz AE, et al. Obligate role for ketone body oxidation in neonatal metabolic homeostasis. J Biol Chem. 2011.[PMID NBP1-02780]
- Garbow JR, Doherty JM, Schugar RC, et al. Hepatic steatosis, inflammation, and ER stress in mice maintained long-term on a very low-carbohydrate ketogenic diet. Am J Physiol Gastrointest Liver Physiol. 2011 Mar 31.. [PMID: 21454445]
- Ma D, Tremblay P, Mahngar K et al. A novel synthetic C-1 analogue of 7-deoxypancratistatin induces apoptosis in p53 positive and negative human colorectal cancer cells by targeting the mitochondria: enhancement of activity by tamoxifen. Invest New Drugs. 2011 Apr 15 [PMID: 21494837]
- Wang RC, Levine B. Calcipotriol Induces Autophagy in HeLa Cells and Keratinocytes. J Invest Dermatol. 2011. [PMID 21228817]
- Xu B, Hua J, Zhang Y, et al. Proliferating cell nuclear antigen (PCNA) regulates primordial follicle assembly by promoting apoptosis of oocytes in fetal and neonatal mouse ovaries. PLoS ONE. 2011;6(1):e16046.[PMID 21253613]
- Cotter DG, d'Avignon DA, Wentz AE, et al. Obligate role for ketone body oxidation in neonatal metabolic homeostasis. J Biol Chem. 2011 Jan 5. [PMID: 21209089]
- Wang AM, Morishima Y, Clapp KM, et al. Inhibition of hsp70 by methylene blue affects signaling protein function and ubiquitination and modulates polyglutamine protein degradation. J Biol Chem. 2010 May 21;285(21):15714-23. [PMID: 20348093] (Human, WB)
- Zhou X, Wang L, Hasegawa H et al. Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9424-9.[PMID: 20439739] (Mouse, WB)
- Ben Sahra I, et al. Targeting cancer cell metabolism: the combination of metformin and 2-deoxyglucose induces p53-dependent apoptosis in prostate cancer cells. Cancer Res. 2010 Mar 15;70(6):2465-75. Epub 2010 Mar 9.
- Aguado C, et al. Laforin, the most common protein mutated in Lafora disease, regulates autophagy. Hum Mol Genet. 2010 Jul 15;19(14):2867-76. Epub 2010 May 7.(Mouse, WB)
- Eng CH, et al. Ammonia derived from glutaminolysis is a diffusible regulator of autophagy. Sci Signal. 2010 Apr 27;3(119):ra31.
- Choi JY, et al. The role of autophagy in follicular development and atresia in rat granulosa cells. Fertil Steril. 2010 May 15;93(8):2532-7. Epub 2010 Feb 10.
- Cao Y, Marks JD, Marks JW, et al. Construction and characterization of novel, recombinant immunotoxins targeting the Her2/neu oncogene product: in vitro and in vivo studies. Cancer Res. 2009 Dec 1;69(23):8987-95. [PMID: 19934334]
- Ferguson CJ, Lenk GM, Meisler MH. Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2. Hum Mol Genet. 2009 Dec 15;18(24):4868-78. [PMID: 19793721] (Mouse, WB)
- Fu L, Kim YA, Wang X, et al. Perifosine inhibits mammalian target of rapamycin signaling through facilitating degradation of major components in the mTOR axis and induces autophagy. Cancer Res. 2009 Dec 1;69(23):8967-76. [PMID: 19920197]
- Tang D, Kang R, Xiao W, et al. Quercetin prevents LPS-induced high-mobility group box 1 release and proinflammatory function. Am J Respir Cell Mol Biol. 2009 Dec;41(6):651-60. [PMID: 19265175]
- Woloszynek JC, Kovacs A, Ohlemiller KK, et al. Metabolic Adaptations to Interrupted Glycosaminoglycan Recycling. J Biol Chem 2009;284(43):29684-29691. [PMID: 19700765]
- Yan T, Seo Y, Kinsella TJ. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res. 2009 Nov 15;15(22):6912-20. [PMID: 19861440]
- Fan S, Meng Q, Saha T, et al. Low Concentrations of Diindolylmethane, a Metabolite of Indole-3-Carbinol, Protect against Oxidative Stress in a BRCA1-Dependent Manner. Cancer Res 2009;69(15):6083-6091. [PMID: 19622773]
- Dreux M, Gastaminza P, Wiel SF, et al. The autophagy machinery is required to initiate hepatitis C virus replication. PNAS 2009;106(33):14046-14051. [PMID: 19666601] (Human, WB)
- Huttenhower C, Haley EM, Hibbs MA, et al. Exploring the human genome with functional maps. Genome Res 2009;19(6):1093-1106. [PMID: 19246570] (Human, WB)
- Shen S, Zhang P, Lovchik MA, et al. Cyclodepsipeptide toxin promotes the degradation of Hsp90 client proteins through chaperone-mediated autophagy. J Cell Biol 2009;185(4):629-639. [PMID: 19433452] (Human, ICC/IF)
- Sarkar S, Korolchuk V, Renna M, et al. Methodological considerations for assessing autophagy modulators: a study with calcium phosphate precipitates. Autophagy. 2009 Apr;5(3):307-13. [PMID: 19182529]
- Zhu Y, Vergote D, Pardo C, et al. CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy. FASEB J 2009:fj.08-128819 [PMID: 19380511]
- Huang J, Canadien V, Lam GY, et al. Activation of antibacterial autophagy by NADPH oxidases. PNAS 2009;106(15):6226-6231 [PMID: 19339495] (Mouse, WB)
- Pacheco CD, Elrick MJ, Lieberman AP. Tau deletion exacerbates the phenotype of Niemann-Pick type C mice implicates autophagy in pathogenesis. Hum Mol Genet 2009;18(5):956-965 [PMID: 19074461] (Human, Mouse, WB)
- Panyasrivanit M, Khakpoor A, Wikan N, et al. Co-localization of constituents of the dengue virus translation replication machinery with amphisomes. J Gen Virol. 2009 Feb;90(Pt 2):448-56. [PMID: 19141455] (Human, WB)
- Yuan H, Perry CN, Huang C, et al. LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection. Am J Physiol Heart Circ Physiol. 2009 Feb;296(2):H470-9. [PMID: 19098111] (WB, IF)
- Humbey O, Pimkina J, Zilfou JT, et al. The ARF tumor suppressor can promote the progression of some tumors. Cancer Res. 2008 Dec 1;68(23):9608-13. [PMID: 19047137] (Mouse, WB)
- Young JE, Martinez RA, La Spada AR. Nutrient deprivation induces neuronal autophagy and implicates reduced insulin signaling in neuroprotective autophagy activation. J Biol Chem. 2009 Jan 23;284(4):2363-73. [PMID: 19017649] (Mouse, WB, IHC/IF)
- Degtyarev M, De Mazière A, Orr C, et al. Akt inhibition promotes autophagy and sensitizes PTEN-null tumors to lysosomotropic agents. J Cell Biol. 2008 Oct 6;183(1):101-16. [PMID: 18838554] (Transfected, WB)
- Wong ES, Tan JM, Soong WE, et al. Autophagy-mediated clearance of aggresomes is not a universal phenomenon. Hum Mol Genet. 2008 Aug 15;17(16):2570-82. [PMID: 18502787] (Transfected, ICC/IF)
- Burdette DL, Yarbrough ML, Orvedahl A, et al. Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, then cell lysis. PNAS 2008;105(34):12497-12502. [PMID: 18713860] (Mouse, WB)
- Miller BC, Zhao Z, Stephenson LM, et al. The autophagy gene ATG5 plays an essential role in B lymphocyte development. Autophagy. 2008 Apr 1;4(3):309-14. [PMID: 18188005]
- Zhang H, Bosch-Marce M, Shimoda LA, et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem. 2008 Apr 18;283(16):10892-903. [PMID: 18281291] (Mouse, Western Blot)
- Settembre C, Fraldi A, Jahreiss L, et al. A block of autophagy in lysosomal storage disorders. Hum Mol Genet. 2008 Jan 1;17(1):119-29. [PMID: 17913701] (Mouse, IF)
- Sarkar S, Krishna G, Imarisio S, et al. A rational mechanism for combination treatment of Huntington's disease using lithium and rapamycin. Hum Mol Genet. 2008 Jan 15;17(2):170-8. [PMID: 17921520] (Transfected, WB)
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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| LC3 Antibody [DyLight 488] | NB100-2220G | Hu, Mu, Rt | IHC, IHC-P, IP, WB | | (19) | | | | LC3 Antibody [DyLight 550] | NB100-2220R | Hu, Mu, Rt | IHC, IHC-P, IP, WB | | (19) | | | | LC3 Antibody [DyLight 650] | NB100-2220C | Hu, Mu, Rt | IHC, IHC-P, IP, WB | | (19) | | | | LC3 Antibody [Biotin] | NB100-2220B | Hu, Mu, Rt | IHC, IHC-P, IP, WB | (2) | (4) | | | | LC3 Antibody [HRP] | NB100-2220H | Hu, Mu, Rt | IHC, IHC-P, IP, WB | (2) | (4) | | |
Positive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| LC3B Lysate | NBL1-12844 | | WB | (1) | | | | Other Positive Controls- Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies
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Antibody Packs (1)Lysates (3)Peptides and ProteinsPrimary Antibodies (25)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| LC3 Antibody | NB100-2331 | Bv, Hu, Mu, Rt, Xp, Ze | IHC-P, IP, WB | (1) | (14) | | | | LC3 Antibody | NBP1-19167 | Bv, Hu, Mu, Rt, Ze | FACS, IHC-P, WB | (3) | (1) | | | | LC3 Antibody | R-146-100 | Hu, Rt | IF, IHC-P, WB | (9) | (1) | | | | LC3 Antibody (EP1983Y) | NB110-57179 | Hu, Mu, Rt | FACS, ICC, IHC-P, IP, WB | (3) | (2) | | | | LC3 Antibody (EP1528Y) | NB110-57180 | Hu, Mu, Rt | ICC, IHC-P, IP, WB | (2) | (2) | | | | ... and 20 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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