LAMP-1/CD107a Antibody 0.025 ml Late Endosome Marker

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Ordering Information: NBP1-49668

  • Catalog Number
    NBP1-49668
  • Displayed Format
    Unconjugated
  • Size(s)
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    LAMP-1/CD107a Antibody Summary

    Species Human, Mouse, Rat (Negative)
    Tested Applications ICC/IF, IHC, IHC-P
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    LAMP1
    Purity
    Immunogen affinity purified
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    LAMP-1/CD107a Antibody Details

    Marker
    Late Endosome Marker
    Immunogen
    A genomic peptide made to an internal region of the human LAMP1 protein (within residues 250-400). [Swiss-Prot P11279]
    Localization
    Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.Nucleus

    Species Reactivity

    Human and mouse. Antibody was negative in rat.

    Applications/Dilutions

    Dilutions
    • Immunocytochemistry/Immunofluorescence 1:50-1:100
    • Immunohistochemistry 1:100
    • Immunohistochemistry-Paraffin 1:100
    Application Notes
    This LAMP1 antibody is useful for ICC/IF and IHC. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

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    Product PDFs

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    Notes

    Manufactured by Genomic Antibody Technology™. GAT FAQs

    Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS
    Unit Size
    0.025 ml
    Concentration
    1.19 mg/ml
    Preservative
    0.05% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol LAMP1
    Entrez
    Uniprot

    Alternate Names for LAMP-1/CD107a Antibody

    • CD107 antigen-like family member A
    • CD107a
    • CD107a antigen
    • LAMP-1
    • LAMPA
    • LGP120
    • lysosomal-associated membrane protein 1
    • lysosome-associated membrane glycoprotein 1
    • Lysosome-associated membrane protein 1
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    Related Products by Gene

    Background

    LAMP1 is a type I transmembrane protein integral in the membranes of endosomes and lysosomes. It is proposed to function in cell-cell interactions. It is important in transporting newly synthesized molecules from the trans-Golgi network at the plasma membrane. Certain components of the LAMP1 protein are sufficient to confer proper intracellular targeting. In all, appropriate sorting and transport of molecules is critical in tumor genesis, and metastasis formation.

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    There are no publications for LAMP-1/CD107a Antibody (NBP1-49668).
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    Product General Protocols

    View specific protocols for LAMP-1/CD107a Antibody (NBP1-49668): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

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    FAQs for LAMP-1/CD107a (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)