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Ki67 Antibody 0.1ml Proliferation Marker

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Catalog Number  NB500-170
Sample Size Available
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Jump to Information on Ki67 Antibody (NB500-170)

Ki67 Antibody Summary
 
Species Human, Mouse, Rat, Porcine
Tested Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality Polyclonal
Host Rabbit
Gene MKI67
Purity Immunogen affinity purified
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Ki67 Antibody Details
 
Marker Proliferation Marker
Immunogen A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]
Localization Nuclear
Species Reactivity
 
Human, mouse, rat (pubmed 22521325) and porcine.
Reviewed Species Read 1 rated 4  using NB500-170 in the following species:
 
Publications Read 14 using NB500-170 in the following species:
 
Applications/Dilutions
 
Dilutions
  • Immunocytochemistry/Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
Application Notes

This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot; however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Positive Controls

1 Positive Control
Directly Conjugated
Formats
Biotin Labeled NB500-170B
DyLight 550 Labeled NB500-170R
DyLight 488 Labeled NB500-170G
DyLight 650 Labeled NB500-170C
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 4  using
NB500-170 in the following application:
Publications
Read 14 publications using
NB500-170 in the following applications:
Product PDFs
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Reviews
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Publications
Ki67 Antibody (NB500-170) has been mentioned in at least 27 publications.
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Protocols and FAQs
Controls & Support Products
View all related Ki67 Controls and Support Products.
Research Tools
View all Ki67 Research Tools.
Packaging, Storage & Formulations
 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer PBS, 30% glycerol
Unit Size 0.1ml (also available in 0.025ml (sample size))
Concentration 1.11 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 6 months from date of receipt.
Bioinformatics
 
Gene Symbol MKI67
Entrez
Uniprot
Alternate Names for Ki67
 
  • proliferation-related Ki-67 antigen
  • antigen KI-67
  • KIA
  • antigen identified by monoclonal Ki-67
Related Products by Gene
 
KI-67, TP53, CDKN1A, PCNA, PGR, BCL2, EGFR, ESR1, KI67, ERBB2, CCND1, TMEM37, PRH2, PAK3, TCEAL1, NSG1, MMP9, VIM, DCX, TNF
Background
 
Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. It interacts with KIF15 as well as MKI67IP, and is suggested to be involved in cell cycle regulation. Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.