Ki67 Antibody 0.1 ml Proliferation Marker

Ki67 NB500-170

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Ordering Information: NB500-170

Catalog NumberNB500-170
  • FormUnconjugated
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Ki67 Antibody Summary

 
Species Human, Mouse, Rat, Porcine
Tested Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality Polyclonal
Host Rabbit
Gene MKI67
Purity Immunogen affinity purified
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Ki67 Antibody Details

 
Isotype IgG
Marker Proliferation Marker
Immunogen A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]
Localization Nuclear

Species Reactivity

 
Human, mouse, rat (pubmed 22521325) and porcine.
Reviewed Species Read 2 rated 4.5  using NB500-170 in the following species:
 
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Applications/Dilutions

 
Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
Application Notes

This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot; however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).

Positive Controls

1 Positive Control

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB500-170AF405
Alexa Fluor (R) 488 Labeled NB500-170AF488
Alexa Fluor (R) 647 Labeled NB500-170AF647
Alexa Fluor (R) 700 Labeled NB500-170AF700
Biotin Labeled NB500-170B
DyLight 405 Labeled NB500-170V
DyLight 488 Labeled NB500-170G
DyLight 550 Labeled NB500-170R
DyLight 650 Labeled NB500-170C
FITC Labeled NB500-170F
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 4  using
NB500-170 in the following application:
Publications
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NB500-170 in the following applications:

Product PDFs

Reviews

Average Rating: 4.5 
Recent Reviews

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Publications

Ki67 Antibody (NB500-170) has been mentioned in at least 39 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all Ki67 Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer PBS, 30% glycerol
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1.03 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol MKI67
Entrez
Uniprot

Alternate Names for Ki67

 
  • proliferation-related Ki-67 antigen
  • antigen KI-67
  • KIA
  • antigen identified by monoclonal Ki-67

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Background

 
Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. It interacts with KIF15 as well as MKI67IP, and is suggested to be involved in cell cycle regulation. Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.