Ki-67/MKI67 Antibody 0.1 ml Proliferation Marker

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Western Blot: Ki67 Antibody [NB500-170] - Western blot analysis of A431 (A) and Hek293 (B) cell lysate using Ki67 antibody (NB500-170) at 2 ug/ml.
Immunocytochemistry/Immunofluorescence: Ki67 Antibody [NB500-170] - Confocal immunofluorescent analysis of MCF7 cells using Ki67 antibody (NB500-170, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Ki67 Antibody [NB500-170] - Human tonsil.
Immunocytochemistry/Immunofluorescence: Ki67 Antibody [NB500-170] - Ki67 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunocytochemistry/Immunofluorescence: Ki67 Antibody [NB500-170] - Ki67 antibody (1:100) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunocytochemistry/Immunofluorescence: Ki67 Antibody [NB500-170] - Ki67 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. A dilution of 1:100 was used.
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Ki-67/MKI67 Antibody Summary

Species Human, Mouse, Rat, Porcine
Tested Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Immunogen affinity purified
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Ki-67/MKI67 Antibody Details

Proliferation Marker
A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]

Species Reactivity

Human, mouse, rat (pubmed 22521325) and porcine.
Reviewed Species Read 3 rated 4.3
using NB500-170 in the following species:

Publications Read Publications using NB500-170 in the following species:


  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
Application Notes
This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot; however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Positive Controls
Other Available
Alexa Fluor (R) 405 Labeled NB500-170AF405
Alexa Fluor (R) 488 Labeled NB500-170AF488
Alexa Fluor (R) 647 Labeled NB500-170AF647
Alexa Fluor (R) 700 Labeled NB500-170AF700
Biotin Labeled NB500-170B
DyLight 405 Labeled NB500-170V
DyLight 488 Labeled NB500-170G
DyLight 550 Labeled NB500-170R
DyLight 650 Labeled NB500-170C
FITC Labeled NB500-170F
Reviewed Applications
Read 2 Review rated 4.3
NB500-170 in the following applications:

Read Publications using
NB500-170 in the following applications:

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Average Rating: 4.3
Recent Reviews
ICC/IF Tested In Human
Reviewed On: 06/09/2014
Rated: 5

Ki-67/MKI67 NB500-170
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Ki-67/MKI67 Antibody (NB500-170) has been mentioned in at least 43 publications.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
Unit Size
0.1 ml
1.03 mg/ml
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.


Gene Symbol MKI67

Alternate Names for Ki-67/MKI67 Antibody

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • KIA
  • proliferation-related Ki-67 antigen
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Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. It interacts with KIF15 as well as MKI67IP, and is suggested to be involved in cell cycle regulation. Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.

Reviews for Ki-67/MKI67 Antibody (NB500-170) (3) 4.33

Read what people are saying who have used Ki-67/MKI67 Antibody (NB500-170).

We have 3 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2: Immunocytochemistry/Immunofluorescence, Western Blot.
Average Rating: 4.3
(Based on 3 reviews)

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
Human Anonymous - 12/29/2014


Sample TestedSee PMID 19582877
Immunocytochemistry/Immunofluorescence Ki-67/MKI67 NB500-170
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ICC/IF Human Anonymous 06/09/2014


Special ApplicationsConfocal immunofluorescent analysis of MCF7 cells using Ki67 antibody (NB500-170, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
  Very Good
WB Mouse Anonymous 09/30/2010


ApplicationWestern Blot
Sample TestedWhole cell lysate

Publications for Ki-67/MKI67 Antibody (NB500-170) (43)

Read what people are writing who have used Ki-67/MKI67 Antibody (NB500-170).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.

Have you published using Ki-67/MKI67 Antibody (NB500-170)? Submit your review and earn rewards points which can be used for merchandise & discounts.
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Dai G, Freudenberger T, Zipper P et al. Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases. Am J Pathol, 2007;171(5):1451-61. 2007 November 1. [PMID: 17982124] (IHC) IHC
Kim SS, Cao L, Baek HJ et al. Impaired skin and mammary gland development and increased gamma-irradiation-induced tumorigenesis in mice carrying a mutation of S1152-ATM phosphorylation site in Brca1. Cancer Res;69(24):9291-300. 2009 Dec 15. [PMID: 19996295]
Zhao Z. Endoplasmic reticulum stress in maternal diabetes-induced cardiac malformations during critical cardiogenesis period. Birth Defects Res B Dev Reprod Toxicol. 2011 Sep 15. [PMID: 21922638] (Mouse) Mouse
Chen F, Loturco J. A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition. J Neurosci Methods. 2012 Apr 11. [PMID: 22521325] (IHC, Rat) IHC Rat
Ip, M et al. The t10,c12 isomer of conjugated linoleic acid stimulates mammary tumorigenesis in transgenic mice over-expressing erbB2 in the mammary epithelim. Carcinogenesis; 28: 1269 - 1276. 2007 June. [PMID: 17259656]
Gerdes, J et al. Immunobiochemical molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am J Pathol 138: 867-73. 1991 [PMID: 2012175]
Knott, AW et al. Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection. Am J Physiol Gastrointest Liver Physiol 287: G562-70. 2004 [PMID: 15142831]
Zecchini, V et al. Notch signaling regulates the differentiation of post-mitotic intestinal epithelial cells. Genes Dev 19: 1686-91. 2005 [PMID: 16024658]
Micci, MA et al. Caspase inhibition increases survival of neural stem cells in the gastrointestinal tract. Neurogastroenterol Motil 17: 557-64. 2005 [PMID: 16078945]
Kelly, P et al. THe G12 family of heterotrimeric G proteins promotes breast cancer invasion metastasis. PNAS 103:8173-8178. 2006 [PMID: 16705036]
Ge, R et al. Inhibition of Growth Metastasis of Mouse Mammary Carcinoma by Selective Inhibitor of Transforming Growth Factor-{beta} Type I Receptor Kinase In vivo. Clin, Canc Res 12: 315-4330. 2006 [PMID: 16857807] (IHC-P, Mouse) IHC-P Mouse
Kojima, Kyoko et al. Inactivation of Smad4 Accelerates Kras G12D-Mediated Pancreatic Neoplasia. Cancer Res 67: 8121-8130. 2007 [PMID: 17804724]
Nam, S Y, J S Kim et al. DA-6034, a Derivative of Flavonoid, Prevents Ameliorates Dextran Sulfate Sodium-Induced Colitis Inhibits Colon Carcinogenesis. Experimental Biology Medicine 233: 180-191. 2008 [PMID: 18222973]
Olofsson, P S, L A Soderstrom et al. CD137 Is Expressed in Human Atherosclerosis Promotes Development of Plaque Inflammation in Hypercholesterolemic Mice. Circulation: CIRCULATIONAHA107.699173. 2008 [PMID: 18285570]
ampa VM, Gutierrez-Lanza R, Cerignoli F et al. Notch activates cell cycle reentry progression in quiescent cardiomyocytes. J Cell Biol;183(1):129-141. 2008 [PMID: 18838555]
Gaur S, Chen L, Yen T et al. Preclinical study of the cyclodextrin-polymer conjugate of camptothecin CRLX101 for the treatment of gastric cancer Nanomedicine 2011 Oct 25 [PMID: 22033079] (IHC, Mouse) IHC Mouse
McCaskey SJ, Rondini EA, Langohr IM, Fenton JI. Differential effects of energy balance on experimentally-induced colitis World J Gastroenterol 2012 Feb 21 [PMID: 22363133] (IHC, Mouse) IHC Mouse
Rock K, Meusch M, Fuchs N et al. Estradiol Protects Dermal Hyaluronan/Versican Matrix during Photoaging by Release of Epidermal Growth Factor from Keratinocytes J Biol Chem 2012 Jun 8 [PMID: 22493503] (IHC-Fr, Mouse) IHC-Fr Mouse
Wu X, Zhang Q, Wang X et al. Survivin Is Required for Beta-Cell Mass Expansion in the Pancreatic Duct-Ligated Mouse Model plos One 2012 [PMID: 22870272] (IHC, Mouse) IHC Mouse
Rauhavirta T, Oittinen M, Kivisto R et al. Are Transglutaminase 2 Inhibitors Able to Reduce Gliadin-Induced Toxicity Related to Celiac Disease? A Proof-of-Concept Study J Clin Immunol 2012 Aug 10 [PMID: 22878839] (IHC-Fr, Human) IHC-Fr Human
Zhang Y, Srinivasan B, Xing C, Lu J. A New Chalcone Derivative (E)-3-(4-Methoxyphenyl)-2-methyl-1-(3,4,5-trimethoxyphenyl)prop-2-en-1-one Suppresses Prostate Cancer Involving p53-mediated Cell Cycle Arrests and Apoptosis Anticancer Res 2012 Sep [PMID: 22993307]
Lee NJ, Choi DY, Song JK et al. Deficiency of C-C chemokine receptor 5 suppresses tumor development via inactivation of NF-{kgreen}B and inhibition of monocyte chemoattractant protein-1 in urethane-induced lung tumor model Carcinogenesis 2012 Oct 5 [PMID: 22907530] (IHC, Mouse) IHC Mouse
Tao YM, Huang JL, Zeng S et al. BTB/POZ domain-containing protein 7: Epithelial-mesenchymal transition promoter and prognostic biomarker of hepatocellular carcinoma. Hepatology 2013 Jan 16 [PMID: 23325674] (IHC-P, Human, Mouse) IHC-P Human, Mouse
Imamura F, Greer CA. Pax6 regulates Tbr1 and Tbr2 expressions in olfactory bulb mitral cells. Mol Cell Neurosci 2013 Jan 22 [PMID: 23353076] (IHC-Fr, ICC/IF, Mouse) ICC/IF, IHC-Fr Mouse
Mildner M, Hacker S, Haider T et al. Secretome of Peripheral Blood Mononuclear Cells Enhances Wound Healing PLoS One 2013 [PMID: 23533667] (IHC-P, ICC/IF, Human) ICC/IF, IHC-P Human
Saggar JK, Fung AS, Patel KJ, Tannock IFet al. Use of molecular biomarkers to quantify the spatial distribution of effects of anticancer drugs in solid tumors. Mol Cancer Ther 2013 Apr [PMID: 23348047] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Kirby ED, Muroy SE, Sun WG et al. Acute stress enhances adult rat hippocampal neurogenesis and activation of newborn neurons via secreted astrocytic FGF2. Elife 2013 Apr 16 [PMID: 23599891] (IHC-Fr, ICC/IF, Rat) ICC/IF, IHC-Fr Rat
Biddlestone-Thorpe L, Sajjad M, Rosenberg E et al. ATM Kinase Inhibition Preferentially Sensitizes p53-Mutant Glioma to Ionizing Radiation. Clin Cancer Res 2013 Jun 15 [PMID: 23620409] (IHC-Fr, ICC/IF, Human) ICC/IF, IHC-Fr Human
Maeda N, Kokai Y, Ohtani S et al. Anti-tumor effect of orally administered spinach glycolipid fraction on implanted cancer cells, colon-26, in mice. Lipids 2008 Aug [PMID: 18594894] (IHC-P, Mouse) IHC-P Mouse
Spiegelberg L, Braks J, Djasim U et al. Effects of hyperbaric oxygen therapy on the viability of irradiated soft head and neck tissues in mice. Oral Dis 2013 Jul 8 [PMID: 23865954] (IHC-P, Mouse) IHC-P Mouse
Xiang L, Gilkes DM, Chaturvedi P, Semenza GL et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265]
Liu Y, Sanchez-Tillo E, Lu X et al. Rb1 family mutation is sufficient for sarcoma initiation. Nat Commun. 2013 Oct 23 [PMID: 24150016] (ICC/IF, Mouse) ICC/IF Mouse
Niedworok C, Rock K, Kretschmer I et al. Inhibitory Role of the Small Leucine-Rich Proteoglycan Biglycan in Bladder Cancer. PLoS One. 2013 Nov 6 [PMID: 24223213] (IHC, Human) IHC Human
Saggar JK, Tannock IF. Activity of the hypoxia-activated pro-drug TH-302 in hypoxic and perivascular regions of solid tumors and its potential to enhance therapeutic effects of chemotherapy. Int J Cancer. 2013 Nov 8 [PMID: 24338277] (IHC-Fr, ICC/IF, Human) ICC/IF, IHC-Fr Human
Kwon S, Kim MS, Lee ES, Park JK. A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells. Integr Biol (Camb) 2014 Mar 5 [PMID: 24599518] (IHC-P, ICC/IF, Human) ICC/IF, IHC-P Human
Gou HF, Huang J, Shi HS, Wang YS. Chemo-immunotherapy with oxaliplatin and interleukin-7 inhibits colon cancer metastasis in mice. PLoS ONE 2014 Jan 27 [PMID: 24465710] (IHC-P, Mouse) IHC-P Mouse
Sano M, Sasaki T, Hirakawa S et al. Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm. PLoS ONE 3/21/2014 [PMID: 24651519] (IHC-P, Human) IHC-P Human
Sherman-Baust CA, Kuhn E, Valle BL et al. A genetically engineered ovarian cancer mouse model based on fallopian tube transformation mimics human high-grade serous carcinoma development. J. Pathol. 3/20/2014 [PMID: 24652535] (IHC-P, Mouse) IHC-P Mouse
Takano N, Sarfraz Y, Gilkes DM et al. Decreased Expression of Cystathionine b-Synthase Promotes Glioma Tumorigenesis. Mol. Cancer Res. 2014 Jul 03 [PMID: 24994751] (IHC-P, Human)

Ki67 antibody used for IHC-P on tumor xenografts derived from injection of parental U87-MG cells and shCBS#07 subclone (Fig 1E, Fig 3D).
IHC-P Human
Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (IHC-P, Human) IHC-P Human
Xie H, Cui Z, Wang L et al. PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis. Nat. Med. 2014 Oct 05 [PMID: 25282358] (IHC, Mouse) IHC Mouse
Uegaki T, Taniguchi F, Nakamura K et al. Inhibitor of apoptosis proteins (IAPs) may be effective therapeutic targets for treating endometriosis Hum. Reprod. 2014 Nov 05 [PMID: 25376458] (IHC-P, Mouse) IHC-P Mouse
Kawanishi Y, Nakasa T, Shoji T et al. Intra-articular injection of synthetic microRNA-210 accelerates avascular meniscal healing in rat medial meniscal injured model. Arthritis Res. Ther. 2014 Nov 28 [PMID: 25430980] (IHC-P, Rat)

Ki67 antibody used for IHC-P in Spraque Dawley rats subjected to intra-articular injection of double-stranded miR-210 (for control group using control dsRNA) with atelocollagen administered immediately after injury (injured meniscal healing in a rat model) - 4%PFA fixation, 4um sections, blocking with horse serum, Ki67 antibody diluted at 1:100 (Figure 4).

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FAQs for Ki-67/MKI67 (4)

  1. Could you please let us know if you have the antibody which may have cross-reactivity to hamster tissue in IHC(paraffin or frozen)? Also, please tell us reference information using your products to hamster tissue.
    • Thank you for your patience. The only information I was able to gain is that the protein has 61% homology with the chinese hamster. Our lab only recommends use if the % homology is 85% or above so I would not recommend our Ki67 antibodies for your customer. However if they are willing to still test this they would become eligable for our Innovators Reward Program in which they would gain a 50% refund and a 50% discount on a future purchase. All you would need to do is email us at Here is the link to all of our Ki67 antibodies.
  2. What is the IF protocol which was used to obtain the IF image on the datasheet?
    • Our lab used our general ICC protocol to obtain the image seen on our website.
  3. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  4. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)