KRAS Antibody (H00003845-M01)

KRAS Antibody (3B10-2F2)

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KRAS Antibody (3B10-2F2) Summary:
Species:Hu
Applications:ELISA, IF, WB
Clonality:Monoclonal
Gene:KRAS
Purity:IgG purified
Host:Mouse
Specificity:KRAS - v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
View Additional KRAS Products
KRAS Antibody (3B10-2F2) Details:
Clone:3B10-2F2
Isotype:IgG1 Kappa
Immunogen:KRAS (AAH13572, 1 a.a. ~ 189 a.a) full length recombinant protein with GST tag.
Epitope:MTEYKLVVVGAGGVGKSALTIQLIQNHFVD EYDPTIEDSYRKQVVIDGETCLLDILDTAG HEEYSAMRDQYMRTGEGFLCVFAINNTKSF EDIHHYREQIKRVKDSEDVPMVLVGNKCDL PSRTVDTKQAQDLARSYGIPFIETSAKTRQ GVDDAFYTLVREIRKHKEKMSKDGKKKKKK SKTKCVIM*
Species Reactivity:

Human. Other species not tested.

Applications:
Uses:Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Dilutions:ELISA, immunofluorescence, Western Blot 1:500
Unit Size:0.1 mg
Concentration:Please see the vial label for concentration.
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:In 1x PBS, pH 7.2
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: KRAS
Entrez3845 (Human)
OMIM109800, 114480,
Swiss ProtAAH13572 (Human)
 
Background:

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

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Working with KRAS

KRAS Antibody (3B10-2F2) Images (3)
Immunofluorescence of monoclonal antibody to KRAS (H00003845-M01) on HeLa cell . [antibody concentration 10 ug/ml]KRAS monoclonal antibody (M01), clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.
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Publications (1)

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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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KRAS in Context


Research Areas related to KRAS (4):
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