Integrin alpha M/CD11b Antibody (EPR1344) 0.1 ml Microglia Marker, Myeloid Marker

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Western Blot: Integrin alpha M/CD11b Antibody (EPR1344) [NBP1-42075] - Mouse perfipheral nerve and ganglia visualized with a 1:1000 dilution. Image provided by verified customer review.
Immunohistochemistry-Paraffin: Integrin alpha M/CD11b Antibody (EPR1344) [NBP1-42075] - Human spleen tissue.
Western Blot: Integrin alpha M/CD11b Antibody (EPR1344) [NBP1-42075] - (A) THP-1 +TPA (B) TF-1 cell lysates.
Immunohistochemistry-Paraffin: Integrin alpha M/CD11b Antibody (EPR1344) [NBP1-42075] - Analysis of paraffin-embedded human tonsil tissue using anti-CD11b.
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Ordering Information: NBP1-42075

  • Catalog Number
    NBP1-42075
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 ml
  • Availability
  • Price
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Integrin alpha M/CD11b Antibody (EPR1344) Summary

Species Human, Mouse, Rat (Negative)
Tested Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Monoclonal
Host
Rabbit
Gene
ITGAM
Purity
Protein A purified
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Integrin alpha M/CD11b Antibody (EPR1344) Details

Clone
EPR1344
Isotype
IgG
Marker
Microglia Marker, Myeloid Marker
Immunogen
A synthetic peptide corresponding to residues in human CD11b was used as an immunogen.

Species Reactivity

For Mouse this antibody is recommended for Western Blot only.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-5000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
Application Notes
In Western blot this antibody detects a band at approximately 170 kDa.

Contact Information

Product PDFs

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Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS 59%, Glycerol 40%, BSA 0.05%, pH: 7.40
Unit Size
0.1 ml
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Preservative
0.01% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol ITGAM
Entrez
Uniprot

Alternate Names for Integrin alpha M/CD11b Antibody (EPR1344)

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b
  • CD11b antigen
  • CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
  • Cell surface glycoprotein MAC-1 subunit alpha
  • CR-3 alpha chain
  • CR3AMGC117044
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • Leukocyte adhesion receptor MO1
  • MAC-1
  • MAC1A
  • macrophage antigen alpha polypeptide
  • MO1A
  • Neutrophil adherence receptor
  • neutrophil adherence receptor alpha-M subunit
  • SLEB6
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Related Products by Gene

Background

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Integrin alpha M/CD11b (3)

  1. I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
    • I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for Integrin alpha M/CD11b

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