Integrin alpha 6/CD49f Antibody (NKI-GoH3) [Biotin] 0.1 mg

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Flow Cytometry: Integrin alpha 6 Antibody (NKI-GoH3) [Biotin] [NB100-63904] Staining of human peripheral blood platelets with Rat anti Human CD49f:Biotin
Flow Cytometry: Integrin alpha 6 Antibody (NKI-GoH3) [Biotin] [NB100-63904] - Staining of human peripheral blood lymphocytes.
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Ordering Information: NB100-63904

  • Catalog Number
    NB100-63904
  • Displayed Format
    Biotin
  • Other Formats
  • Size(s)
    0.1 mg
  • Availability
  • Price
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Integrin alpha 6/CD49f Antibody (NKI-GoH3) [Biotin] Summary

Species Human, Mouse, Canine, Primate
Tested Applications FLOW
Clonality
Monoclonal
Host
Rat
Gene
ITGA6
Purity
Protein G purified
Specificity
NB100-63904 recognizes CD49F (VLA-6 alpha chain), a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (7).
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Integrin alpha 6/CD49f Antibody (NKI-GoH3) [Biotin] Details

Format
Biotin
Clone
NKI-GoH3
Isotype
IgG2a
Immunogen
BALB/c mouse mammary tumour cells

Species Reactivity

Cross reacts with Cynomolgus monkey, Monkey, Dog, Human and Mouse.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Positive Controls
Other Available
Formats
FITC Labeled NB100-63905
PE Labeled NB100-63906
Readout System

Contact Information

Product PDFs

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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline pH7.4
Unit Size
0.1 mg
Concentration
0.1 mg/ml
Preservative
0.09% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol ITGA6
Entrez
Uniprot

Alternate Names for Integrin alpha 6/CD49f Antibody (NKI-GoH3) [Biotin]

  • CD49 antigen-like family member F
  • CD49f
  • CD49f antigen
  • DKFZp686J01244
  • FLJ18737
  • integrin alpha-6
  • integrin alpha6B
  • integrin, alpha 6
  • ITGA6B
  • VLA-6
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Related Products by Gene

Background

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.

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FAQs for Integrin alpha 6/CD49f (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Positive Control Lysate(s)

Other Available Formats

FITC Labeled NB100-63905
PE Labeled NB100-63906

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