IRS2 Antibody (NSB828)
IRS2 [phospho Ser731] Antibody
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anti-Insulin Receptor Substrate 2 antibody, anti-IRS 2 antibody, |
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IRS2 [phospho Ser731] Antibody Summary: | | Gene: | IRS2 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This is specific for human IRS 2 phosphorylated at the position of Serine 731. The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared IRS peptides. It only recognizes the phosphorylated serine 731, and does not cross-react with other phosphorylated sites. |
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| Publications: | Antibody has at least 8 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional IRS2 Products IRS2 [phospho Ser731] Antibody Details: | Isotype: | IgG |
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| Immunogen: | Synthetic peptide (YKASpSPAESS) corresponding to amino acids 727-736 of human IRS 2 at the phosphorylated site of Serine 731. This synthetic peptide also correlates to amino acids 129-138 of mouse IRS 2 at the phosphorylation sites of Serine 133 and to amino acids 229-238 of rat IRS 2 at the serinephosphorylation site 233. |
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| Localization: | Cytoplasmic |
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| Modification: | phospho Ser731 |
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Cross-reacts with Human. Not yet tested in other species. |
| Uses: | Immunoprecipitation, Western Blotting and ELISA
This antibody detects a band of approximately 200 kDa in cell lysate derived from mouse serum-treated fibroblasts, which corresponds to the predicted molecular weight of phospho IRS 2. Samples were boiled prior to subject 7.5% SDS-polyacrylamide gel electrophoresis. |
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| Dilutions: | ELISA 0.1 - 1.0 ug/ml,
immunoprecipitation 3.0 - 5.0 ug,
Western Blot 0.5 - 2.0 ug/ml |
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| Unit Size: | 0.2 ml |
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| Concentration: | 0.5 mg/ml |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | PBS |
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| Preservative: | 0.02% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, IRS 4. IRS 2, a cytoplasmic signaling molecule that mediates effects of insulin, Insulin-like growthfactor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has adual function, serving either for a positive or negative modulation of insulin signal transduction.
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Working with IRS2
IRS2 [phospho Ser731] Antibody Images (0)
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Publications (8)
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Jump to: Peptides and Proteins, Primary Antibodies, Secondary Antibodies
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Related ProductsPeptides and ProteinsPrimary Antibodies (13)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| IRS2 [p Ser731] Antibody | NBP1-72215 | Hu, Mu, Rt | ELISA, IHC-P, IP, WB | (2) | (1) | | | | IRS2 Antibody | NBP1-96563 | Hu, Mu | FACS, IHC-P, WB | (3) | | | | | IRS2 Antibody (EP903Y) | NB110-57138 | Hu, Mu(-), Rt(-) | ICC, IP, WB | (2) | (1) | | | | IRS2 Antibody | NBP1-74482 | Hu, Mu, Rt | ELISA, IHC-P, IP, WB | (2) | (1) | | | | IRS2 Antibody | NB100-68242 | Hu | IP, WB | (1) | | | | | ... and 8 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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