Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residue 1 and 50 of human motif containing GTPase activating protein 1 using the numbering given in entry NP_003861.1 (GeneID 8826).
Species Reactivity:
Human
Applications:
Uses:
This antibody is useful for western blot, Immunohistochemistry- and immunoprecipitation.
Dilutions:
Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation 2-5 mcg/mg lysate, Western Blot 1:2000-1:10000
Unit Size:
0.1 ml
Concentration:
0.2 mg/ml
Notes:
Western Blot Image Notes: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti- IQGAP1 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. IQGAP1 was also immunoprecipitated by another rabbit anti-IQGAP1 antibody which recognizes a downstream epitope.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-buffered Saline containing 0.1% BSA
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq]
Immunofluorescence: IQGAP1 Antibody [NBP1-06529] FFPE of human breast carcinoma. Affinity purified rabbit anti-IQGAP1 1:100. Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 1:100.
Western Blot and Immunoprecipitation: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate from HeLa and 293T cells. IQGAP1 was also immunoprecipitated by another rabbit anti-IQGAP1 antibody.