NB100-63052 binds to the C-terminal 122 amino acids of mKIAA0051, also known as Murine IQ motif containing GTPase activating protein 1.The mKIAA0051 protein (IQGAP1) has a fundamental role in cell motility and invasion. It binds to activated CDC42 but does not stimulate its GTPase activity. It also associates with calmodulin and regulates cadherin-based cell adhesion. Potentially mKIAA0051 serves as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. HCA008 may bind to the human Ras GTPase-activating-like protein IQGAP1 (p195), due to 98% sequence identity in the antigen region.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Fusion protein of a domain of murine mKIAA0051 (amino acid residues 1536-1675, MW 14 kDa) expressed in E. coli.
Species Reactivity:
Mouse. Not yet tested in other species.
Applications:
Uses:
This antibody is useful for ELISA, Immunohistochemistry-Frozen, Western Blot
Dilutions:
ELISA
Immunohistochemistry-Frozen
Western Blot
Unit Size:
0.1 mg
Concentration:
Please see the vial label for concentration.
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Preservative:
Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Western Blot: KIAA0051 Antibody (1260) [NB100-63052] - analysis of various adult mouse tissues showing a band at approx. 191kDa when probed with 0.5ug/ml NB100-63052 followed by Goat anti Human IgG F(ab')2: HRP
Immunohistochemistry-Frozen: KIAA0051 Antibody (1260) [NB100-63052] - Staining of frozen sections from 10 week-old ICR mice stained with 2ug/ml KIAA0051
Ohara, R. et al. (2006) Antibodies for proteomic research: comparison of traditional immunization with recombinant antibody technology. Proteomics. 6: 2638-2646.
Mataraza, J. M. et al. (2003) IQGAP1 promotes cell motility invasion. J. Biol. Chem. 278: 41237-41245.