Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide, C-RYKVDYESQSTDTQN, representing the of the sequence according to NP_001013416.1; NP_000589.2
Species Reactivity:
Human, Mouse, Rat, Dog
Applications:
Uses:
This antibody is useful for Peptide ELISA and Western Blot. Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Dilutions:
Peptide ELISA 1:2000, Western Blot 1:1000
Unit Size:
0.1 mg
Concentration:
0.5 mg/ml
Packaging:
Storage:
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
Tris-saline [pH7.3] with 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
NBP1-00203 (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.