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Mouse IGFBP-1 ELISA Kit

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      BEK-2031-2P
    • Availability
      Product Discontinued

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Mouse IGFBP-1 ELISA Kit Summary

Specificity
Mouse Insulin-like growth factor-binding protein 1
Kit Type
ELISA Kit
Gene
IGFBP1

Applications/Dilutions

Dilutions
  • ELISA 31.2pg/ml-2000pg/ml
Application Notes
This product is useful for Sandwich ELISA detection. Strip format Biosensis ELISA kits offer the highest level of sensitivity available Strip 96-well format, use only what you need: High sensitivity and low blank Suitability for serum & tissues Reproducible results This kit contains: Pre-coated plates, Prepared standards Buffers, Detection reagents, Substrate Precise methods/instructions.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Notes

This kit contains 2.0 x 96.0 Well Strip.

Alternate Names for Mouse IGFBP-1 ELISA Kit

  • alpha-pregnancy-associated endometrial globulin
  • amniotic fluid binding protein
  • binding protein-25
  • binding protein-26
  • binding protein-28
  • growth hormone independent-binding protein
  • hIGFBP-1
  • IBP1
  • IBP-1
  • IGF-binding protein 1
  • IGFBP1
  • IGFBP-1
  • IGF-BP25
  • insulin-like growth factor binding protein 1
  • insulin-like growth factor-binding protein 1
  • Placental protein 12
  • PP12AFBP

Background

The mouse Insulin-like growth factor-binding protein 1 (IGFBP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IGFBP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IGFBP-1 concentration are added to these wells and allowed to complex with the bound IGFBP-1 antibody. A biotinylated mouse IGFBP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGFBP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IGFBP-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IGFBP-1 ELISA Kit (BEK-2031-2P). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol IGFBP1