IGFBP1 Antibody (NB120-10732)
IGFBP1 Antibody (33627.11)
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anti-AFBP antibody, IGFBP 1 antibody, anti-PP12 antibody, anti-Placental protein 12 antibody, anti-Insulin Like Growth Factor Binding Protein 1 antibody, anti-IGF BP25 antibody, anti-IGF binding protein 1 antibody, anti-IBP1 antibody, anti-IBP 1 antibody, anti-iBinding protein 28 antibody, anti-hIGFBP1 antibody, anti-hIGFBP 1 antibody, anti-Growth hormone independent binding protein antibody, anti-Binding protein 26 antibody, anti-Binding protein 25 antibody, |
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IGFBP1 Antibody (33627.11) Summary: | | Gene: | IGFBP1 |
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| Purity: | Protein G purified |
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| Host: | Mouse |
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| | Specificity: | No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4. |
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| Publications: | Antibody has at least 3 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional IGFBP1 Products IGFBP1 Antibody (33627.11) Details: | Clone: | 33627.11 |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant full length human insulin-like growth factor binding protein 1, expressed in Escherichia coli. |
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| Localization: | Secreted |
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Cross-reacts with Human. Not yet tested in other species. |
| Uses: | ELISA: Use at a concentration of 2 ug/ml. In the ELISA capture assay, plates are coated with 100 ul/well of the capture antibody (2 ug/ml) in combination with 100 ul/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained. Neut: Use at a concentration of 10 - 40 ug/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 ug/ml in the presence of approximately 5 ug/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells. WB: Use at a concentration of 1-2 ug/ml. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions. ot tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
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| Dilutions: | ELISA 2 ug/ml,
Western Blot 1-2 ug/ml,
Neut |
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| Unit Size: | 0.1 mg |
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| Concentration: | 0.5 mg/ml |
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| Positive Controls: | 2 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | PBS |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Insulin-like growth factor binding protein-1 (IGFBP-1) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). The IGFBP members are cysteine-rich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxyterminal regions of the molecule. Contained within IGFBP-1 and
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Reviews (0) Related Products (18) Working with IGFBP1
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Working with IGFBP1
IGFBP1 Antibody (33627.11) Images (0)
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Publications (3)IGFBP1 Antibody (33627.11) Background References:- Karey, K.P., et al., Cancer Research, 48, 4083
- Jones, J.I., Clemmons, D.R., Endocrine Rev., 16, 3 (1995).
more... - Kelly, K.M., et al., Insulin-like growth factor-binding proteins (IGFBPs) their regulatory dynamics. Int. J. Biochem. Cell Biol., 28, 619-637 (1996).
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Novus Explorer for IGFBP1 gene
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