Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Peptide with sequence C-KQQEQKYPQGVASQR, from the C Terminus of the protein sequence according to NP_006539.3; NP_001007226.1.
Epitope:
C-KQQEQKYPQGVASQR
Species Reactivity:
Human, Mouse, Dog
Applications:
Uses:
Peptide ELISA and Western Blot. Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293.
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
Tris-saline [pH7.3] with 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Western Blot: IGF2BP2 Antibody [NB100-93408] - (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: IGF2BP2 Antibody [NB100-93408] - HEK293 over expressing IGF2BP2 (RC205673) and probed with NB100-93408 (mock transfection in first lane), tested by Origene.
Zeggini E, et al. Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes. Science. 2007 Jun 1;316(5829):1336-41. Epub 2007 Apr 26. pubmed: 17463249 [PubMed - in process]