IGF2BP2 Antibody (NB100-93408)

IGF2BP2 Antibody

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IGF2BP2 Antibody Summary:
Species:Ca, Hu, Mu
Applications:PEP-ELISA
Clonality:Polyclonal
Gene:IGF2BP2
Purity:Immunogen affinity purified
Host:Goat
Specificity:This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1).
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
View Additional IGF2BP2 Products
IGF2BP2 Antibody Details:
Isotype:IgG
Immunogen:Peptide with sequence C-KQQEQKYPQGVASQR, from the C Terminus of the protein sequence according to NP_006539.3; NP_001007226.1.
Epitope:C-KQQEQKYPQGVASQR
Species Reactivity:

Human, Mouse, Dog

Applications:
Uses:Peptide ELISA and Western Blot. Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293.
Dilutions:Peptide ELISA 1:8000
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris-saline [pH7.3] with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: IGF2BP2
Entrez10644 (Human)
319765 (Mouse)
Swiss ProtNP_006539.3; NP_001007226.1
 
Background:

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

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Working with IGF2BP2

IGF2BP2 Antibody Images (2)
Western Blot: IGF2BP2 Antibody [NB100-93408] - (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescenceWestern Blot: IGF2BP2 Antibody [NB100-93408] - HEK293 over expressing IGF2BP2 (RC205673) and probed with NB100-93408 (mock transfection in first lane), tested by Origene.
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Publications (1)

IGF2BP2 Antibody Background References:

  1. Zeggini E, et al. Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes. Science. 2007 Jun 1;316(5829):1336-41. Epub 2007 Apr 26. pubmed: 17463249 [PubMed - in process]

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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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IGF2BP2 in Context


Research Areas related to IGF2BP2 (5):
Novus Explorer for IGF2BP2 gene

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