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IGF-II/IGF2 Partial Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43176
    • Availability
      Product Discontinued

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IGF-II/IGF2 Partial Protein Summary

Description
A partial recombinant protein of Human IGF-2.

Source: E. coli
Protein/Peptide Type
Partial Protein
Gene
IGF2

Applications/Dilutions

Dilutions
  • Immunoassay
Application Notes
This reagent has been tested in bioassays using the MCF-7 cell line. The ED50 measured in the dose-dependent induction of MCF-7 cell proliferation assay is typically 0.85 ng/ml, corresponding to a specific activity of approximately 1 x10^6 Units/mg. Purity > 97%, as determined by SDS-PAGE
Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 97%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: The ED50 measured in the dose-dependent induction of MCF-7 cell proliferation assay is typically 0.85 ng/ml, corresponding to a specific activity of approximately 1 x106 Units/mg.

Alternate Names for IGF-II/IGF2 Partial Protein

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGFII
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A

Background

Insulin-like growth factor II (IGF-2), also known as somatomedin A, is a member of the insulin-like growth factor family expressed in many cell types and is secreted into the blood. IGF-2 is a potent mitogen and plays an important role in embryonic development. IGF-2 also plays important roles in obesity and tumor pathogenesis. IGF-1 and IGF-2 share 70% sequence identity. IGF-2 is 100% identical among human, bovine and porcine and exhibits cross-species activity. Recombinant Human IGF-2 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7.5 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGF2