IGF2 Antibody (NB600-1122)

IGF2 Antibody

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IGF2 Antibody Summary:
Species:Hu
Applications:ELISA, Func, WB
Clonality:Polyclonal
Gene:IGF2
Purity:Affinity purified
Host:Rabbit
Specificity:Rabbit polyclonal antibody specific for Human Insulin Like Growth Factor-II (IGF-II). These polyclonal antibodies were raised against highly purified recombinant human IGF-2.
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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IGF2 Antibody Details:
Immunogen:Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
Localization:Secreted
Species Reactivity:

Cross-reacts with Human. Not yet tested in other species.

Applications:
Uses:NEUTRALIZING ACTIVITY:concentration of 4.5 ug/mL required for half-maximal inhibition of 10.0 ng/mL IGF-II. ELISA: Antibody concentration of 0.5 ug/mL (100 uL per well) to detect 0.4 ng/well human IGF-II. WESTERN BLOT: 0.2 ug/mL to detect 1.5 - 3.0 ng/lane IGF-II under both reducing and non-reducing conditions
Dilutions:ELISA 1:2000, Functional Neutralizing Activity, Western Blot 1:1000
Unit Size:0.1 mg
Concentration:Lyoph
Notes:
Add diH20 for desired concentration.
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:Lyophilized in PBS, pH 7.4. Reconstitute with 100µl of distilled water.
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: IGF2
Entrez3481 (Human)
 
Background:

Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types

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Working with IGF2

IGF2 Antibody Images (1)
Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa Lane 1: Marker
Lane 2: 250 ng Lane 3: 125 ng
Lane 4: 62.5 ng Lane 5: 31.25 ng
Lane 6: 15.625 ng Lane 7: 7.8 ng
Lane 8: 3.9 ng
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Publications (1)

IGF2 Antibody Product Specific:

  1. Wilson EM et al. Autocrine growth factor signaling by insulin-like growth factor-II mediates MyoD-stimulated myocyte maturation. J Biol Chem 278:41109-13 (2003).

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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, Secondary Antibodies

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IGF2 in Context


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