Rabbit polyclonal antibody specific for Human Insulin Like Growth Factor-II (IGF-II). These polyclonal antibodies were raised against
highly purified recombinant human IGF-2.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
Localization:
Secreted
Species Reactivity:
Cross-reacts with Human. Not yet tested in other species.
Applications:
Uses:
NEUTRALIZING ACTIVITY:concentration of 4.5 ug/mL required for half-maximal inhibition of
10.0 ng/mL IGF-II.
ELISA: Antibody concentration of 0.5 ug/mL (100 uL per well) to detect
0.4 ng/well human IGF-II.
WESTERN BLOT: 0.2 ug/mL to detect 1.5 - 3.0 ng/lane IGF-II under both
reducing and non-reducing conditions
Dilutions:
ELISA 1:2000, Functional Neutralizing Activity, Western Blot 1:1000
Unit Size:
0.1 mg
Concentration:
Lyoph
Notes:
Add diH20 for desired concentration.
Packaging:
Storage:
Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:
Lyophilized in PBS, pH 7.4. Reconstitute with 100µl of distilled water.
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa Lane 1: Marker Lane 2: 250 ng Lane 3: 125 ng Lane 4: 62.5 ng Lane 5: 31.25 ng Lane 6: 15.625 ng Lane 7: 7.8 ng Lane 8: 3.9 ng