Goat anti-Human IgM Heavy Chain Secondary Antibody [DyLight 549] Summary
| Immunogen |
Human IgM u chain |
| Specificity |
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. |
| Isotype |
IgM |
| Clonality |
Polyclonal |
| Host |
Goat |
| Gene |
IGHM |
| Purity |
Immunogen affinity purified |
Applications/Dilutions
| Dilutions |
- Fluorophore-linked immunosorbent assay 1:20000
- Immunocytochemistry/ Immunofluorescence 1:5000
- Western Blot 1:10000
|
| Application Notes |
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free |
| Preservative |
0.01% Sodium Azide |
| Concentration |
LYOPH |
| Purity |
Immunogen affinity purified |
| Reconstitution Instructions |
Please contact technical support for detailed reconstituion instruction. |
Notes
Emission Wavelength: 568, Excitation Wavelength: 550, Label Molecular Weight: 982, Restore with 0.1 mL of deionized water (or equivalent).
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for Goat anti-Human IgM Heavy Chain Secondary Antibody [DyLight 549]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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