Histone H3 [p Thr11, p Ser10] Antibody 0.025 mg Nuclear Marker

Histone H3 NB21-1092

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Ordering Information: NB21-1092

Catalog NumberNB21-1092
  • FormUnconjugated
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Histone H3 [p Thr11, p Ser10] Antibody Summary

 
Species Human, Mouse, C. elegans
Predicted Species Rat (100%), Chicken (100%), Drosophila (100%), Plant (100%), Xenopus (100%). Backed by our 100% guarantee.
Tested Applications WB, ChIP, DB, ICC/IF
Clonality Polyclonal
Host Rabbit
Gene HIST2H3C
Purity Immunogen affinity purified
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward Test in a species/application not listed above to receive a full credit towards a future purchase.
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Histone H3 [p Thr11, p Ser10] Antibody Details

 
Marker Nuclear Marker
Modification p Thr11, p Ser10
Immunogen Synthetic phosphorylated peptides surrounding Serine 10 and Threonine 11 of human Histone H3.2 [Swiss Prot Q71DI3].
Localization Nucleus. Chromosome.

Species Reactivity

 
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Applications/Dilutions

 
Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:200
Application Notes

This Histone H3 pS10/pT11 antibody is useful for ChIP*, Dot Blot, ICC/IF, and Western Blot where a band is seen ~15 kDa in HeLa histone preps and C. elegans embryo lysate.

Positive Controls

6 Positive Controls

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB21-1092AF405
Alexa Fluor (R) 488 Labeled NB21-1092AF488
Alexa Fluor (R) 647 Labeled NB21-1092AF647
Alexa Fluor (R) 700 Labeled NB21-1092AF700
Biotin Labeled NB21-1092B
DyLight 405 Labeled NB21-1092V
DyLight 488 Labeled NB21-1092G
DyLight 550 Labeled NB21-1092R
DyLight 650 Labeled NB21-1092C
FITC Labeled NB21-1092F
HRP Labeled NB21-1092H
Learn about Custom Antibody Labeling
Control Peptide
Histone H3 Peptide (NB21-1092PEP)

Product PDFs

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Protocols and FAQs

Controls & Support Products

Research Tools

View all Histone H3 Research Tools.

Notes

 
0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane. Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer PBS, 30% glycerol
Unit Size 0.025 mg (also available in 0.05 mg)
Concentration 1.2 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol HIST2H3C
Entrez
Uniprot

Alternate Names for Histone H3

 
  • H3.3B
  • MGC87782
  • histone H3.3
  • H3.3AH3F3H3F3B
  • MGC87783
  • H3 histone, family 3A

Related Products by Gene

Background

 
When confronted with DNA damage or the lack of certain kinases like Chk1 and Aurora1, histone H3 is phosphorylated at T11, causing a reduction in acetylation of the same histone at K9 and ultimately leading to a reduction of active transcription in affected cells. When the T11 phosphorylation is combined with a typical S10 phosphorylation that is critical in chromosome condensation in metaphase, the effect on transcriptional regulation is amplified further. Phosphorylation of H3S10 is decreased by T11 phosphorylation. Thus, detection of the combined modification is critical in determining transcriptional effects, especially when contrasted against single modifications of H3.