Histone H3 [p Thr11, p Ser10] Antibody 0.025 mg Nuclear Marker

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Western Blot: Histone H3 [p Ser10, p Thr11] Antibody [NB21-1092] - WB analysis of h3pS10/pT11 in C. elegans embryo lysate.
Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr11, p Ser10] Antibody [NB21-1092] - ICC staining of H3pS10/pT11 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Western Blot: Histone H3 [p Ser10, p Thr11] Antibody [NB21-1092] - WB detection of H3pS10/pT11 in HeLa histone preps.
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Ordering Information: NB21-1092

  • Catalog Number
    NB21-1092
  • Displayed Format
    Unconjugated
  • Other Formats
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Histone H3 [p Thr11, p Ser10] Antibody Summary

Species Human, Mouse, C. elegans
Predicted Species
Rat (100%), Chicken (100%), Drosophila (100%), Xenopus (100%), Plant (100%). Backed by our 100% Guarantee
Tested Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
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Histone H3 [p Thr11, p Ser10] Antibody Details

Marker
Nuclear Marker
Modification
p Thr11, p Ser10
Immunogen
Synthetic phosphorylated peptides surrounding Serine 10 and Threonine 11 of human Histone H3.2 [Swiss Prot Q71DI3].
Localization
Nucleus. Chromosome.

Species Reactivity

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:200
Application Notes
This Histone H3 pS10/pT11 antibody is useful for ChIP*, Dot Blot, ICC/IF, and Western Blot where a band is seen ~15 kDa in HeLa histone preps and C. elegans embryo lysate.
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB21-1092AF405
Alexa Fluor (R) 488 Labeled NB21-1092AF488
Alexa Fluor (R) 647 Labeled NB21-1092AF647
Alexa Fluor (R) 700 Labeled NB21-1092AF700
Biotin Labeled NB21-1092B
DyLight 405 Labeled NB21-1092V
DyLight 488 Labeled NB21-1092G
DyLight 550 Labeled NB21-1092R
DyLight 650 Labeled NB21-1092C
FITC Labeled NB21-1092F
HRP Labeled NB21-1092H
Control Peptide
Histone H3 Peptide (NB21-1092PEP)

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Product PDFs

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Notes

0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane. Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 mg
Concentration
1.2 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot

Alternate Names for Histone H3 [p Thr11, p Ser10] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • histone H3.3
  • MGC87782
  • MGC87783
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Related Products by Gene

Background

When confronted with DNA damage or the lack of certain kinases like Chk1 and Aurora1, histone H3 is phosphorylated at T11, causing a reduction in acetylation of the same histone at K9 and ultimately leading to a reduction of active transcription in affected cells. When the T11 phosphorylation is combined with a typical S10 phosphorylation that is critical in chromosome condensation in metaphase, the effect on transcriptional regulation is amplified further. Phosphorylation of H3S10 is decreased by T11 phosphorylation. Thus, detection of the combined modification is critical in determining transcriptional effects, especially when contrasted against single modifications of H3.

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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1092): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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FAQs for Histone H3 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Bioinformatics Tool for Histone H3

Discover related pathways, diseases and genes to Histone H3. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Research Areas for Histone H3

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Blogs on Histone H3

Check out the latest blog posts on Histone H3.
Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

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Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

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Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that ...  Read full blog post.

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