Histone H3 [p Thr11, p Ser10] Antibody Summary
||Human, Mouse, C. elegans
Rat (100%), Chicken (100%), Drosophila (100%), Xenopus (100%), Plant (100%). Backed by our 100% Guarantee
||WB, ChIP, DB, ICC/IF
Immunogen affinity purified
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Histone H3 [p Thr11, p Ser10] Antibody Details
p Thr11, p Ser10
Synthetic phosphorylated peptides surrounding Serine 10 and Threonine 11 of human Histone H3.2 [Swiss Prot Q71DI3].
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
- Western Blot 1:500
- Chromatin Immunoprecipitation 2-5 ug per million cells
- Dot Blot 1:1000
- Immunocytochemistry/Immunofluorescence 1:200
This Histone H3 pS10/pT11 antibody is useful for ChIP*, Dot Blot, ICC/IF, and Western Blot where a band is seen ~15 kDa in HeLa histone preps and C. elegans embryo lysate.
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0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane. Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for Histone H3 [p Thr11, p Ser10] Antibody
- H3 histone, family 3A
- histone H3.3
Related Products by Gene
, HISTONE H3
When confronted with DNA damage or the lack of certain kinases like Chk1 and Aurora1, histone H3 is phosphorylated at T11, causing a reduction in acetylation of the same histone at K9 and ultimately leading to a reduction of active transcription in affected cells. When the T11 phosphorylation is combined with a typical S10 phosphorylation that is critical in chromosome condensation in metaphase, the effect on transcriptional regulation is amplified further. Phosphorylation of H3S10 is decreased by T11 phosphorylation. Thus, detection of the combined modification is critical in determining transcriptional effects, especially when contrasted against single modifications of H3.
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FAQs for Histone H3 (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
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