0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane. Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
H3K9 methylations are a conserved hallmark of heterochromatin binding domains. The trimethyl K9 version of histone H3, when coupled with a T6 phosphorylation, is related to the Oct4 and Nanog proteins in embryoid bodies. This association seems to indicate a role for this modified histone in cellular differentiation in development. The formation of this modification seems to be a result of a complicated multiple silencing process for downstream genes, which is RNAi independent.
Reviews for Histone H3 Antibody (NB21-1054) (0)
There are no reviews for Histone H3 Antibody (NB21-1054).
By submitting a review earn points towards our Rewards Program.
250 points for product review
500 additional points for an image with your product review
Double points (500) if you are the first to review this product
Double points (1000) if you are the first to review this product with an image
Have you used Histone H3 Antibody (NB21-1054)? Submit your review and earn rewards points which can be used for merchandise & discounts.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
FAQs for Histone H3 (2)
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.