Histone H3 [p Thr6] Antibody Summary
||Human, Mouse, C. elegans
Rat (100%), Chicken (100%), Drosophila (100%), Xenopus (100%), Plant (100%). Backed by our 100% Guarantee
||WB, ChIP, DB, ICC/IF
Immunogen affinity purified
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Histone H3 [p Thr6] Antibody Details
Synthetic peptide surrounding Threonine 6 of human Histone H3.2 [Swiss Prot Q71DI3].
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
- Western Blot 1:500
- Chromatin Immunoprecipitation 2-5 ug per million cells
- Dot Blot 1:100-1:2000
- Immunocytochemistry/Immunofluorescence 1:200
This Histone H3 pT6 antibody is useful for ChIP*, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15kDa in HeLa histone prep and C. elegans embryo lysate.
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0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane. Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for Histone H3 [p Thr6] Antibody
- H3 histone, family 3A
- histone H3.3
Related Products by Gene
, HISTONE H3
Phosphorylation as a post-translational modification on histone H3 modulates the ability of proteins to recognize and bind to the H3 tail. When additional modifications to H3 are present, in addition to the native T6 phosphorylation, there is an amplified effect. Several authors suggest that the complexity of these combined PTMs on histone tails should be described as a convoluted language, rather than as a strict code. Since the H3T6 phosphorylation seems to be constitutively present, its de-phosphorylation could be highly significant and affect transcription, repair and replication.
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FAQs for Histone H3 (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for Histone H3
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