Histone H3 [ac Lys4] Antibody Summary
||Human, Mouse, C. elegans
Rat (100%), Chicken (100%), Plant (100%), Drosophila (100%), Xenopus (100%). Backed by our 100% Guarantee
||WB, ChIP, DB, ICC/IF
Immunogen affinity purified
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Histone H3 [ac Lys4] Antibody Details
Synthetic acetylated peptide surrounding Lysine 4 of human Histone H3.2 [Swiss Prot Q71DI3].
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Read 1 rated 5 using NB21-1024 in the following species:
- Western Blot 1:500
- Chromatin Immunoprecipitation 2-5 ug per million cells
- Dot Blot 1:1000
- Immunocytochemistry/Immunofluorescence 1:1000
This H3 K4ac antibody is useful for ChIP, ICC/IF, Western Blot, and Dot Blot. In Western Blot a band is seen ~15-16 kDa in HeLa histone prep, NIH 3T3 histione prep, and C. elegans embryo lysate.
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NB21-1024 in the following applications:
Frequently Purchased Together
WB Tested In Human
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0.05 mg of HeLa histone preps will be included in the shipment along with the primary antibody as an appropriate positive control. For SDS-PAGE with the positive control we recommend loading 0.01 mg per lane.
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for Histone H3 [ac Lys4] Antibody
- H3 histone, family 3A
- histone H3.3
Related Products by Gene
, HISTONE H3
In transcription, H3K4Ac is prevalent at the promoters of active genes. Usually, this modification peaks in the cell cycle after dimethylation of H3K9 occurs, which indicates that there are sequential actions of these two modifications. Methylation that occurs on H3K4 concurrently with acetylation seems to act as an adjuster to the activation effects of acetylation. Shugoshin protein cannot bind to the centromere of active cells when H3K4 is acetylated, which reduces dimethylation, and thus slows meiosis and mitosis. Usually, H3K4Ac is a transitional modification, and will become further modified with methylation as transcription progresses, indicating complex transcriptional regulation.
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We have 1 review tested in 1: Western Blot.
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|Sample Tested||Human breast cell lysate (MDA-MB-231)|
|Dilution Ratio||1:500, 4C O/N|
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FAQs for Histone H3 (2)
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for Histone H3
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